Cloned (Comment) | Organism |
---|---|
overexpression in host strain | Escherichia coli |
General Stability | Organism |
---|---|
dithiothreitol increases thermal stability | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
SDS-PAGE, His-Tag contributed with 3900 Da | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | Escherichia coli | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | Escherichia coli | involved in synthesis of peptidoglycan and lipopolysaccharide | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | Escherichia coli | enzyme of the Leloir pathway | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.4 | - |
purified enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 1 month, 10% loss of activity | Escherichia coli |
25°C, 1 mM dithiothreitol, 1 mM N-acetyl-D-glucosamine 1-phosphate, 20h, 30% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Escherichia coli | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | involved in synthesis of peptidoglycan and lipopolysaccharide | Escherichia coli | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | enzyme of the Leloir pathway | Escherichia coli | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44.3 | - |
UDP-N-acetyl-D-glucosamine | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Escherichia coli |