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Literature summary for 2.7.7.18 extracted from

  • Olland, A.M.; Underwood, K.W.; Czerwinski, R.M.; Lo, M.C.; Aulabaugh, A.; Bard, J.; Stahl, M.L.; Somers, W.S.; Sullivan, F.X.; Chopra, R.
    Identification, characterization, and crystal structure of Bacillus subtilis nicotinic acid mononucleotide adenylyltransferase (2002), J. Biol. Chem., 277, 3698-3707.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21 with a His-tag Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of the apo-enzyme belong to space group: P21 with 58.5% solvent and contain four molecules of NaMN AT in the asymmetric subunit, crystals in complex with deamido-NAD+ belong to space group: P212121 with 55.6% solvent and contain six molecules of NaMN AT in the asymmetric subunit, conditions: polyethylene glycol 3350 and 100 mM MgCl2 Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
NMN
-
Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0068
-
deamido-NAD+ 37┬░C, pH 7.4 Bacillus subtilis
0.043
-
nicotinamide ribonucleotide 37┬░C, pH 7.4 Bacillus subtilis
0.135
-
ATP 37┬░C, pH 7.4 Bacillus subtilis
1.7
-
NAD+ 37┬░C, pH 7.4 Bacillus subtilis
2.3
-
NMN 37┬░C, pH 7.4 Bacillus subtilis
4.2
-
diphosphate 37┬░C, pH 7.4 Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36370 49140 gel filtration, sedimentation equlibrium centrifugation Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.054
-
NMN as substrate Bacillus subtilis
0.34
-
NAD+ as substrate Bacillus subtilis
2.8
-
deamido-NAD+ as substrate Bacillus subtilis
10.1
-
ATP as substrate Bacillus subtilis
11.1
-
nicotinate ribonucleotide as substrate Bacillus subtilis
11.5
-
diphosphate as substrate Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Bacillus subtilis diphosphate + deamido-NAD+
-
r
ATP + NMN
-
Bacillus subtilis diphosphate + NAD+
-
r

Subunits

Subunits Comment Organism
dimer crystallization studies Bacillus subtilis