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Literature summary for 2.7.7.18 extracted from

  • Dahmen, W.; Webb, B.; Preiss, J.
    The deamido-diphosphopyridine nucleotide and diphosphopyridine nucleotide pyrophosphorylases of Escherichia coli and yeast (1967), Arch. Biochem. Biophys., 120, 440-450.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
NAD+ deamido-NAD+ as substrate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
deamido-NAD+ 37┬░C, pH 7.5 Escherichia coli
0.029
-
deamido-NAD+ 37┬░C, pH 7.5 Saccharomyces cerevisiae
0.06
-
ATP 37┬░C, pH 7.5, + nicotinate ribonucleotide Saccharomyces cerevisiae
0.069
-
NAD+ 37┬░C, pH 7.5 Saccharomyces cerevisiae
0.08
-
nicotinate ribonucleotide 37┬░C, pH 7.5 Escherichia coli
0.13
-
nicotinate ribonucleotide 37┬░C, pH 7.5 Saccharomyces cerevisiae
0.2
-
nicotinamide ribonucleotide 37┬░C, pH 7.5 Escherichia coli
0.27
-
3-acetylpyridine-NAD+ 37┬░C, pH 7.5 Saccharomyces cerevisiae
0.37
-
NAD+ 37┬░C, pH 7.5 Escherichia coli
0.4
-
nicotinamide ribonucleotide 37┬░C, pH 7.5 Saccharomyces cerevisiae
0.5
-
ATP 37┬░C, pH 7.5 Escherichia coli
0.65
-
diphosphate 37┬░C, pH 7.5 Saccharomyces cerevisiae
0.74
-
3-pyridinealdehyde-NAD+ 37┬░C, pH 7.5 Saccharomyces cerevisiae
1.1
-
diphosphate 37┬░C, pH 7.5 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activation, 25 mM, NAD+-synthesis, not deamido-NAD+-synthesis Escherichia coli
Mg2+ requirement Escherichia coli
Mg2+ requirement Saccharomyces cerevisiae
NH4Cl activation, can substitute for KCl Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinate ribonucleotide Escherichia coli
-
diphosphate + deamido-NAD+
-
r
ATP + nicotinate ribonucleotide Saccharomyces cerevisiae
-
diphosphate + deamido-NAD+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Saccharomyces cerevisiae
-
brewer's yeast
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli
partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.0003
-
3-acetyl-pyridine-NAD+ as substrate Escherichia coli
0.0008
-
3-pyridinealdehyde-NAD+ as substrate Escherichia coli
0.004
-
NAD+ as substrate Escherichia coli
0.024
-
deamido-NAD+ as substrate Escherichia coli
1.42
-
3-pyridinealdehyde-NAD+ as substrate Saccharomyces cerevisiae
3.82
-
3-acetyl-pyridine-NAD+ as substrate Saccharomyces cerevisiae
3.85
-
deamido-NAD+ as substrate Saccharomyces cerevisiae
5
-
NAD+ as substrate Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
0-3┬░C, 22% loss of activity within 31 days Saccharomyces cerevisiae
0-3┬░C, 31 days stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-acetyl-pyridine-NAD+ poor substrate Escherichia coli ?
-
?
ATP + 3-acetyl-pyridine-NAD+ reaction at 76% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae ?
-
?
ATP + 3-pyridinealdehyde-NAD+ poor substrate Escherichia coli ?
-
?
ATP + 3-pyridinealdehyde-NAD+ reaction at 28% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae ?
-
?
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide, reverse reaction at 17% the rate of deamido-NAD+-synthesis Escherichia coli diphosphate + NAD+
-
r
ATP + nicotinate ribonucleotide
-
Escherichia coli diphosphate + deamido-NAD+
-
r
ATP + nicotinate ribonucleotide
-
Saccharomyces cerevisiae diphosphate + deamido-NAD+
-
r
ATP + nicotinate ribonucleotide best substrate Escherichia coli diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Escherichia coli diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Saccharomyces cerevisiae diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r
ATP + nicotinate ribonucleotide reaction at 77% the rate of nicotinamide ribonucleotide Saccharomyces cerevisiae diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r
deoxy-ATP + nicotinamide ribonucleotide
-
Escherichia coli ?
-
?
deoxy-ATP + nicotinamide ribonucleotide
-
Saccharomyces cerevisiae ?
-
?
deoxy-ATP + nicotinate ribonucleotide
-
Escherichia coli ?
-
?
deoxy-ATP + nicotinate ribonucleotide
-
Saccharomyces cerevisiae ?
-
?
NAD+ + diphosphate
-
Saccharomyces cerevisiae ATP + nicotinamide nucleotide
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Escherichia coli
37
-
assay at Saccharomyces cerevisiae

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
55
-
5 min 54% loss of activity Escherichia coli
60
-
5 min 39% loss of activity Saccharomyces cerevisiae
60
-
5 min 95% loss of activity Escherichia coli
65
-
5 min 65% loss of activity Saccharomyces cerevisiae