Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and B834 (DE3) cells | Streptomyces wedmorensis |
Crystallization (Comment) | Organism |
---|---|
native enzyme in complex with cytidine 5'-[[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate], hanging drop vapor diffusion method, using 0.1 M HEPES-NaOH (pH 7.5) and 4.5 M NaCl | Streptomyces wedmorensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required for activity. 5 mM used in assay conditions | Streptomyces wedmorensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyethylphosphonate + CTP | Streptomyces wedmorensis | - |
cytidine 5'-[[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate] + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces wedmorensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity column chromatography | Streptomyces wedmorensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyethylphosphonate + CTP | - |
Streptomyces wedmorensis | cytidine 5'-[[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate] + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Fom1 | Fom1 contains a cytidylyltransferase domain at its N-terminus | Streptomyces wedmorensis |
Fom1CyTase | - |
Streptomyces wedmorensis |