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Literature summary for 2.7.7.102 extracted from

  • Ito, N.; Nureki, O.; Yokoyama, S.; Hanaoka, F.
    Crystallization and preliminary x-ray analysis of a DNA primase from hyperthermophilic archaeon Pyrococcus horikoshii (2001), J. Biochem., 130, 727-730 .
    View publication on PubMed
Search for more literature for 2.7.7.102

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
native and selenomethionine-substituted protein, to 1.8 and 2.2 A resolution, respectively, space group P3(2)21 Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O57935 large subunit PriL
-
Pyrococcus horikoshii DSM 12428 O57935 large subunit PriL
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
M13 ssDNA + n dNTP
-
 Pyrococcus horikoshii M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate
-
 ?
M13 ssDNA + n dNTP
-
 Pyrococcus horikoshii DSM 12428 M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 40270, caculated from sequence Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pyrococcus horikoshii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
 poor activity at 60°C Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pyrococcus horikoshii