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Literature summary for 2.7.7.102 extracted from

  • Beck, K.; Vannini, A.; Cramer, P.; Lipps, G.
    The archaeo-eukaryotic primase of plasmid pRN1 requires a helix bundle domain for faithful primer synthesis (2010), Nucleic Acids Res., 38, 6707-6718.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.102

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfolobus islandicus

Crystallization (Commentary)

Crystallization (Comment) Organism
primase domain, hanging drop vapour diffusion technique Sulfolobus islandicus

Protein Variants

Protein Variants Comment Organism
F260A primase activity is similar to wild-type enzyme Sulfolobus islandicus
H141A mutant enzyme without primase and polymerase activity Sulfolobus islandicus
W314A primer formation is severely impaired, very small amount of wild-type like products are formed Sulfolobus islandicus
W347A primase activity is similar to wild-type enzyme Sulfolobus islandicus
W361A primase activity is similar to wild-type enzyme Sulfolobus islandicus
Y352A primase activity is completely abolished Sulfolobus islandicus
Y367A primase activity is similar to wild-type enzyme Sulfolobus islandicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ His141 is involved in the coordination of the zinc ion Sulfolobus islandicus

Organism

Organism UniProt Comment Textmining
Sulfolobus islandicus Q54324 plasmid pRN1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus islandicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information multifunctional replication protein with primase, DNA polymerase and helicase activity. The minimal region required for primase activity encompasses amino-acid residues 40–370 Sulfolobus islandicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Sulfolobus islandicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sulfolobus islandicus