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Literature summary for 2.7.7.102 extracted from
- A novel type of replicative enzyme harbouring ATPase, primase and DNA polymerase activity (2003), EMBO J., 22, 2516-2525.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as a His-tagged protein | Sulfolobus islandicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D111A | the mutant protein is deficient and DNA polymerase activity and primase activity, ATPase activity is unaffected | Sulfolobus islandicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus islandicus | Q54324 | plasmid pRN1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus islandicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 7 dNTP | formation of a ribonucleotide primer when ORF904 is incubated with ribonucleotides and single-stranded M13 DNA. Effcient dNTP incorporation is strongly dependent on the presence of ATP. The non-hydrolysable analogues beta,gamma-imino-ATP and beta,gamma-methylene-ATP are active in stimulation of primer formation. alpha,beta-methylene-ATP does not enhance primer formation. The stimulatory effect is also seen for GTP and UTP but not for ADP or dATP. The majority of the primers have a length of eight nucleotides. Protein ORF904 shows ATPase, DNA polymerase and primase activity | Sulfolobus islandicus | A(pdN)7 + 7 diphosphate | - |
? |  |
| NTP + 7 dNTP | formation of a ribonucleotide primer when ORF904 is incubated with ribonucleotides and single-stranded M13 DNA. Effcient dNTP incorporation is strongly dependent on the presence of ATP. The non-hydrolysable analogues beta,gamma-imino-ATP and beta,gamma-methylene-ATP are active in stimulation of primer formation. alpha,beta-methylene-ATP does not enhance primer formation. The stimulatory effect is also seen for GTP and UTP but not for ADP or dATP. The majority of the primers have a length of eight nucleotides. Protein ORF904 shows ATPase, DNA polymerase and primase activity | Sulfolobus islandicus | N(pdN)7 + 7 diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| protein ORF904 | - |
Sulfolobus islandicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Sulfolobus islandicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Sulfolobus islandicus |
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Release 2023.1 (January 2023)
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