Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4Cl | activation, can substitute for KCl | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | deamide-NAD+ as substrate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
deamido-NAD+ | 37°C, pH 7.5 | Escherichia coli | |
0.029 | - |
deamido-NAD+ | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.06 | - |
ATP | 37°C, pH 7.5, + nicotinate ribonucleotide | Saccharomyces cerevisiae | |
0.067 | 0.069 | NAD+ | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.08 | - |
nicotinate ribonucleotide | 37°C, pH 7.5 | Escherichia coli | |
0.13 | - |
nicotinate ribonucleotide | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.147 | 0.2 | nicotinamide ribonucleotide | - |
Saccharomyces cerevisiae | |
0.147 | 0.2 | nicotinamide ribonucleotide | 37°C, pH 7.5 | Escherichia coli | |
0.27 | - |
3-acetylpyridine-NAD+ | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.37 | - |
NAD+ | 37°C, pH 7.5 | Escherichia coli | |
0.4 | - |
nicotinamide ribonucleotide | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.5 | 0.52 | ATP | 37°C, pH 7.5 | Escherichia coli | |
0.65 | - |
diphosphate | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
0.74 | - |
3-pyridinealdehyde-NAD+ | 37°C, pH 7.5 | Saccharomyces cerevisiae | |
1.1 | - |
diphosphate | 37°C, pH 7.5 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activation, 25 mM, NAD+-synthesis, not deamido-NAD+-synthesis | Escherichia coli | |
Mg2+ | requirement | Escherichia coli | |
Mg2+ | requirement | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | Escherichia coli | - |
diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | Saccharomyces cerevisiae | - |
diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | Escherichia coli B / ATCC 11303 | - |
diphosphate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
B | - |
Escherichia coli B / ATCC 11303 | - |
B | - |
Saccharomyces cerevisiae | - |
brewer's yeast | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
partial | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0038 | - |
NAD+-synthesis | Escherichia coli |
0.004 | - |
NAD+ as substrate | Escherichia coli |
0.024 | - |
deamido-NAD+ as substrate | Escherichia coli |
0.068 | - |
deamido-NAD+-synthesis | Escherichia coli |
3.3 | - |
NAD+-synthesis | Saccharomyces cerevisiae |
3.85 | - |
deamido-NAD+ as substrate | Saccharomyces cerevisiae |
5 | - |
NAD+ as substrate, yeast | Saccharomyces cerevisiae |
7.3 | - |
deamido-NAD+-synthesis | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-acetylpyridine-NAD+ | poor substrate | Escherichia coli | ? | - |
? | |
ATP + 3-acetylpyridine-NAD+ | reaction at 76% the rate of nicotinamide ribonucleotide | Saccharomyces cerevisiae | ? | - |
? | |
ATP + 3-pyridinealdehyde-NAD+ | poor substrate | Escherichia coli | ? | - |
? | |
ATP + 3-pyridinealdehyde-NAD+ | reaction at 28% the rate of nicotinamide ribonucleotide | Saccharomyces cerevisiae | ? | - |
? | |
ATP + nicotinamide ribonucleotide | - |
Escherichia coli | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | - |
Saccharomyces cerevisiae | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | best substrate | Escherichia coli | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | best substrate | Saccharomyces cerevisiae | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | reverse reaction at 17% the rate of deamido-NAD+-synthesis | Escherichia coli | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP | Escherichia coli | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP | Saccharomyces cerevisiae | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Escherichia coli | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Saccharomyces cerevisiae | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | - |
Escherichia coli B / ATCC 11303 | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | best substrate | Escherichia coli B / ATCC 11303 | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | reverse reaction at 17% the rate of deamido-NAD+-synthesis | Escherichia coli B / ATCC 11303 | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP | Escherichia coli B / ATCC 11303 | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Escherichia coli B / ATCC 11303 | diphosphate + NAD+ | - |
r | |
ATP + nicotinate ribonucleotide | best substrate | Escherichia coli | diphosphate + nicotinic acid-adenine dinucleotide | - |
r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Escherichia coli | diphosphate + nicotinic acid-adenine dinucleotide | - |
r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Saccharomyces cerevisiae | diphosphate + nicotinic acid-adenine dinucleotide | - |
r | |
ATP + nicotinate ribonucleotide | reaction at 77% the rate of nicotinamide ribonucleotide | Saccharomyces cerevisiae | diphosphate + nicotinic acid-adenine dinucleotide | - |
r | |
deoxy-ATP + nicotinamide ribonucleotide | - |
Escherichia coli | ? | - |
? | |
deoxy-ATP + nicotinamide ribonucleotide | - |
Saccharomyces cerevisiae | ? | - |
? | |
deoxy-ATP + nicotinate ribonucleotide | reaction at 18% the rate of ATP | Escherichia coli | ? | - |
? | |
deoxy-ATP + nicotinate ribonucleotide | reaction at 30% the rate of ATP | Saccharomyces cerevisiae | ? | - |
? | |
nicotinate mononucleotide + ATP | - |
Escherichia coli | nicotinate adenine dinucleotide + diphosphate | - |
? | |
nicotinate mononucleotide + ATP | - |
Saccharomyces cerevisiae | nicotinate adenine dinucleotide + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5 min 54% loss of activity | Escherichia coli |
60 | - |
5 min 39% loss of activity | Saccharomyces cerevisiae |
60 | - |
5 min 95% loss of activity | Escherichia coli |
65 | - |
5 min 65% loss of activity | Saccharomyces cerevisiae |