Literature summary for 2.7.6.1 extracted from

Donini, S.; Garavaglia, S.; Ferraris, D.M.; Miggiano, R.; Mori, S.; Shibayama, K.; Rizzi, M.
Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis (2017), PLoS ONE, 12, e0175815 .

Search for more literature for 2.7.6.1

Activating Compound

Activating Compound	Comment	Organism	Structure
phosphate	enzyme requires phosphate, optimal concentration ranges from 30 mM to 50 mM	Mycolicibacterium smegmatis

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 0.98 A resolution, structural comparison with human enzyme	Mycolicibacterium smegmatis

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	-	Mycolicibacterium smegmatis
GDP	50% residual activity at 5 mM	Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.058	-	ATP	S0.5 value, Hill coefficient 1.7, pH 7.5, 25°C	Mycolicibacterium smegmatis
0.059	-	D-ribose 5-phosphate	pH 7.5, 25°C	Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	enzyme is allosterically activated by Mg2+, S0.5 is 4mM, Hill coefficient is 2	Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
221000	-	gel filtration	Mycolicibacterium smegmatis

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	A0R3C8	-	-
Mycolicibacterium smegmatis ATCC 700084	A0R3C8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-ribose 5-phosphate	-	Mycolicibacterium smegmatis	AMP + 5-phospho-alpha-D-ribose 1-diphosphate	-	?
ATP + D-ribose 5-phosphate	-	Mycolicibacterium smegmatis ATCC 700084	AMP + 5-phospho-alpha-D-ribose 1-diphosphate	-	?
additional information	diphosphate donors GTP, CTP and UTP are not processed	Mycolicibacterium smegmatis	?	-	-
additional information	diphosphate donors GTP, CTP and UTP are not processed	Mycolicibacterium smegmatis ATCC 700084	?	-	-

Subunits

Subunits	Comment	Organism
hexamer	6 * 35559, calculated from sequence	Mycolicibacterium smegmatis

Synonyms

Synonyms	Comment	Organism
PrsA	-	Mycolicibacterium smegmatis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
77.6	-	D-ribose 5-phosphate	pH 7.5, 25°C	Mycolicibacterium smegmatis
83.5	-	ATP	pH 7.5, 25°C	Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Mycolicibacterium smegmatis

pH Range

pH Minimum	pH Maximum	Comment	Organism
9	-	50% residual activity	Mycolicibacterium smegmatis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.174	-	pH 7.5, 25°C	Mycolicibacterium smegmatis	ADP

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1315	-	D-ribose 5-phosphate	pH 7.5, 25°C	Mycolicibacterium smegmatis
1439	-	ATP	Hill coefficient 1.7, pH 7.5, 25°C	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)