Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M HEPES-Na (pH 7.0), 5% (w/v) Tacsimate, and 10% (w/v) PEG MME 5K | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
D307A | the mutation leads to strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
D320A | the mutation leads to slightly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
D323A | the mutation leads to slightly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
D362A | inactive | Pseudomonas aeruginosa |
D437A | inactive | Pseudomonas aeruginosa |
E304A | the mutation leads to strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
K311A | the mutation leads to strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
K473A | the mutation leads to slightly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
Q416A | the mutation leads to reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R316A | the mutation leads to slightly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R325A | the mutation leads to slightly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R363A | inactive | Pseudomonas aeruginosa |
R419A | inactive | Pseudomonas aeruginosa |
R423A | the mutation leads to strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R477A | the mutation leads to slightly increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
W408A | the mutation leads to strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
Y447A | the mutation leads to reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
(Phosphate)n | wild type enzyme, with GMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
0.055 | - |
(phosphate)3 | wild type enzyme, with GMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
0.062 | - |
(Phosphate)n | wild type enzyme, with AMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
0.11 | - |
(phosphate)3 | wild type enzyme, with AMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
0.82 | - |
AMP | wild type enzyme, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
1.55 | - |
GMP | wild type enzyme, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, most effective metal for activity | Pseudomonas aeruginosa | |
additional information | low activity with Co2+ or Ni2+, no activity in the presence of Mn2+ or Ca2+ | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HYF1 | - |
- |
Pseudomonas aeruginosa DSM 22644 | Q9HYF1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (phosphate)n | - |
Pseudomonas aeruginosa | AMP + (phosphate)n+1 | - |
r | |
ADP + (phosphate)n | - |
Pseudomonas aeruginosa DSM 22644 | AMP + (phosphate)n+1 | - |
r | |
AMP + (phosphate)3 | - |
Pseudomonas aeruginosa | ADP + (phosphate)2 | - |
r | |
AMP + (phosphate)3 | - |
Pseudomonas aeruginosa DSM 22644 | ADP + (phosphate)2 | - |
r | |
AMP + (phosphate)n+1 | - |
Pseudomonas aeruginosa | ADP + (phosphate)n | - |
r | |
AMP + (phosphate)n+1 | - |
Pseudomonas aeruginosa DSM 22644 | ADP + (phosphate)n | - |
r | |
dAMP + (phosphate)n+1 | - |
Pseudomonas aeruginosa | dADP + (phosphate)n | - |
r | |
dAMP + (phosphate)n+1 | - |
Pseudomonas aeruginosa DSM 22644 | dADP + (phosphate)n | - |
r | |
dGMP + (phosphate)n+1 | - |
Pseudomonas aeruginosa | dGDP + (phosphate)n | - |
r | |
dGMP + (phosphate)n+1 | - |
Pseudomonas aeruginosa DSM 22644 | dGDP + (phosphate)n | - |
r | |
GDP + (phosphate)n | the enzyme prefers GDP over ADP | Pseudomonas aeruginosa | GMP + (phosphate)n+1 | - |
r | |
GMP + (phosphate)n+1 | - |
Pseudomonas aeruginosa | GDP + (phosphate)n | - |
r | |
IMP + (phosphate)n+1 | - |
Pseudomonas aeruginosa | IDP + (phosphate)n | - |
r | |
XMP + (phosphate)n+1 | - |
Pseudomonas aeruginosa | XDP + (phosphate)n | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
PA3455 | - |
Pseudomonas aeruginosa |
PPK2d | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
(phosphate)3 | wild type enzyme, with GMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
3.4 | - |
(phosphate)3 | wild type enzyme, with AMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
6.7 | - |
(Phosphate)n | wild type enzyme, with GMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
13.4 | - |
GMP | wild type enzyme, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
37.7 | - |
(Phosphate)n | wild type enzyme, with AMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
44.6 | - |
AMP | wild type enzyme, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | the enzyme is active at pH 8.0-9.5 | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
GMP | wild type enzyme, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
30 | - |
(phosphate)3 | wild type enzyme, with AMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
40 | - |
(phosphate)3 | wild type enzyme, with GMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
50 | - |
AMP | wild type enzyme, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
270 | - |
(Phosphate)n | wild type enzyme, with GMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa | |
610 | - |
(Phosphate)n | wild type enzyme, with AMP as cosubstrate, at pH 8.0 temperature not specified in the publication | Pseudomonas aeruginosa |