Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme L171P, hanging drop vapor diffusion method, 4°C under conditions of 1.22-1.28 M (NH4)2SO4 and 0.1 M Tris-HCl, pH 8.5 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L171P | the mutation dramatically changes the orientation of the LID domain, which can be described as a twisted and closed conformation in contrast to the open and closed conformations in other adenylate kinases | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27144 | - |
- |
Purification (Comment) | Organism |
---|---|
Sepharose column chromatography and Superdex 75 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AMP | - |
Homo sapiens | ADP + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenylate kinase 4 | - |
Homo sapiens |
AK4 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | adenylate kinase 4 is a unique member of the adenylate kinases family which shows no enzymatic activity in vitro | Homo sapiens |