Application | Comment | Organism |
---|---|---|
medicine | characterization of enzyme mutations from patients deficient in red cell adenylate kinase, suffering from chronic hemolytic anemia | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D140del | mutation identified in patient deficient in red cell adenylate kinase, suffering from chronic hemolytic anemia. 30% residual adenylate kinase activity | Homo sapiens |
G40R | mutation identified in patient deficient in red cell adenylate kinase, suffering from chronic hemolytic anemia. 25% residual adenylate kinase activity | Homo sapiens |
R128W | mutation identified in patient deficient in red cell adenylate kinase, suffering from chronic hemolytic anemia. 44% residual adenylate kinase activity | Homo sapiens |
Y164C | mutation identified in patient deficient in red cell adenylate kinase, suffering from chronic hemolytic anemia. 0% residual adenylate kinase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
ATP | mutant G40R, 37°C, pH 8.0 | Homo sapiens | |
0.06 | - |
ATP | mutant G40R, 37°C | Homo sapiens | |
0.07 | - |
AMP | mutant G40R, 37°C, pH 8.0 | Homo sapiens | |
0.07 | - |
AMP | mutant G40R, 37°C | Homo sapiens | |
0.08 | - |
AMP | mutant G64R, 37°C, pH 8.0 | Homo sapiens | |
0.08 | - |
AMP | mutant G64R, 37°C | Homo sapiens | |
0.13 | - |
ATP | wild-type, 37°C | Homo sapiens | |
0.13 | - |
ATP | wild-type, 37°C, pH 8.0 | Homo sapiens | |
0.38 | - |
AMP | wild-type, 37°C | Homo sapiens | |
0.38 | - |
AMP | wild-type, 37°C, pH 8.0 | Homo sapiens | |
0.38 | - |
ATP | mutant G64R, 37°C, pH 8.0 | Homo sapiens | |
0.38 | - |
ATP | mutant G64R, 37°C | Homo sapiens | |
0.4 | - |
ATP | mutant D140del, 37°C, pH 8.0 | Homo sapiens | |
0.4 | - |
ATP | mutant D140del, 37°C | Homo sapiens | |
0.5 | - |
ATP | mutant Y164C, 37°C, pH 8.0 | Homo sapiens | |
0.5 | - |
ATP | mutant Y164C, 37°C | Homo sapiens | |
1.01 | - |
AMP | mutant Y164C, 37°C, pH 8.0 | Homo sapiens | |
1.01 | - |
AMP | mutant Y164C, 37°C | Homo sapiens | |
1.03 | - |
ATP | mutant R11128W, 37°C, pH 8.0 | Homo sapiens | |
1.03 | - |
ATP | mutant R128W, 37°C | Homo sapiens | |
1.6 | - |
AMP | mutant R11128W, 37°C, pH 8.0 | Homo sapiens | |
1.6 | - |
AMP | mutant R128W, 37°C | Homo sapiens | |
1.7 | - |
AMP | mutant D140del, 37°C, pH 8.0 | Homo sapiens | |
1.7 | - |
AMP | mutant D140del, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21635 | - |
x * 22000, SDS-PAGE, x * 21635, calculated | Homo sapiens |
22000 | - |
x * 22000, SDS-PAGE, x * 21635, calculated | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00568 | adenylate kinase-deficient patients with chronic hemolytic anemia | - |
Homo sapiens | P00568 | isoform 1; patients deficient in red cell adenylate kinase, suffering from chronic hemolytic anemia | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2000 | - |
wild-type, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + ATP | - |
Homo sapiens | ADP | - |
? | |
ATP + AMP | - |
Homo sapiens | 2 ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, SDS-PAGE, x * 21635, calculated | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
12 min, wild-type 50% residual activity, mutants G40R, G64R, R128W, D140del loss of more than 90% of initial activity. At 53°C, wild-type is strongly protected by presence of MgATP, mutants are unprotected with a little protection for mutant G40R | Homo sapiens |
53 | - |
half-life 12 min, wild-type. In presence of MgATP, wild-type protein is stringly protected. Mutants G40R, G64R, R128W and D140del are not protected and completely lose activity within 12 min | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
ATP | mutant R11128W, 37°C, pH 8.0 | Homo sapiens | |
0.11 | - |
ATP | mutant G40R, 37°C, pH 8.0 | Homo sapiens | |
0.11 | - |
ATP | mutant G40R, 37°C | Homo sapiens | |
0.16 | - |
AMP | mutant G40R, 37°C, pH 8.0 | Homo sapiens | |
0.16 | - |
AMP | mutant G40R, 37°C | Homo sapiens | |
0.17 | - |
ATP | mutant D140del, 37°C, pH 8.0 | Homo sapiens | |
0.17 | - |
ATP | mutant D140del, 37°C | Homo sapiens | |
0.21 | - |
AMP | mutant D140del, 37°C, pH 8.0 | Homo sapiens | |
0.21 | - |
AMP | mutant D140del, 37°C | Homo sapiens | |
0.57 | - |
AMP | mutant Y164C, 37°C, pH 8.0 | Homo sapiens | |
0.63 | - |
AMP | mutant G64R, 37°C, pH 8.0 | Homo sapiens | |
0.63 | - |
AMP | mutant G64R, 37°C | Homo sapiens | |
0.65 | - |
ATP | mutant G64R, 37°C, pH 8.0 | Homo sapiens | |
0.65 | - |
ATP | mutant G64R, 37°C | Homo sapiens | |
48.32 | - |
ATP | mutant R128W, 37°C | Homo sapiens | |
55.3 | - |
AMP | mutant R11128W, 37°C, pH 8.0 | Homo sapiens | |
55.3 | - |
AMP | mutant R128W, 37°C | Homo sapiens | |
220.3 | - |
ATP | mutant Y164C, 37°C | Homo sapiens | |
234.4 | - |
AMP | mutant Y164C, 37°C | Homo sapiens | |
842 | - |
AMP | wild-type, 37°C, pH 8.0 | Homo sapiens | |
842.3 | - |
AMP | wild-type, 37°C | Homo sapiens | |
879 | - |
ATP | wild-type, 37°C, pH 8.0 | Homo sapiens | |
879.5 | - |
ATP | wild-type, 37°C | Homo sapiens |