BRENDA - Enzyme Database
show all sequences of 2.7.4.20

Two pathways of doolichyl diphosphate dephosphorylation in yeasts

Naumov, A.V.; Shabalin, Y.A.; Vagabov, V.M.; Kulaev, I.S.; Biokhimiya 50, 652-658 (1985)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
-
Saccharomyces cerevisiae
EDTA
1.5 mM, 80% inhibition
Saccharomyces cerevisiae
Ni2+
-
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Saccharomyces cerevisiae
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
requirement, maximal activity at 2 mM
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dolichyl diphosphate + (phosphate)n
Saccharomyces cerevisiae
-
dolichyl phosphate + (phosphate)n+1
-
Saccharomyces cerevisiae
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Storage Stability
Storage Stability
Organism
-10°C, in a stabilizing buffer, over a month with less than 10% loss of activity
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dolichyl diphosphate + (phosphate)n
-
642549
Saccharomyces cerevisiae
dolichyl phosphate + (phosphate)n+1
-
642549
Saccharomyces cerevisiae
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8.5
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
-
Saccharomyces cerevisiae
EDTA
1.5 mM, 80% inhibition
Saccharomyces cerevisiae
Ni2+
-
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Saccharomyces cerevisiae
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
requirement, maximal activity at 2 mM
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dolichyl diphosphate + (phosphate)n
Saccharomyces cerevisiae
-
dolichyl phosphate + (phosphate)n+1
-
Saccharomyces cerevisiae
?
Storage Stability (protein specific)
Storage Stability
Organism
-10°C, in a stabilizing buffer, over a month with less than 10% loss of activity
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dolichyl diphosphate + (phosphate)n
-
642549
Saccharomyces cerevisiae
dolichyl phosphate + (phosphate)n+1
-
642549
Saccharomyces cerevisiae
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8.5
-
Saccharomyces cerevisiae
Other publictions for EC 2.7.4.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
642550
Shabalin
-
Solubilization and properties ...
Saccharomyces pastorianus
Biokhimiya
54
68-73
1989
-
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642549
Naumov
-
Two pathways of doolichyl diph ...
Saccharomyces cerevisiae
Biokhimiya
50
652-658
1985
-
-
-
-
-
-
3
-
1
1
-
1
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
1
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-