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Literature summary for 2.7.4.1 extracted from

  • Rudat, A.K.; Pokhrel, A.; Green, T.J.; Gray, M.J.
    Mutations in Escherichia coli polyphosphate kinase that lead to dramatically increased in vivo polyphosphate levels (2018), J. Bacteriol., 200, e00697-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.1

Application

Application Comment Organism
environmental protection E245K mutation leads to very high polyphosphate accumulation in vivo but is not different from the wild type in either activity or chain length of polyphosphate produced in vitro. Polyphosphate accumulation by bacteria is important in biotechnology applications, e.g. to enhanced biological phosphate removal (EBPR) from wastewater Escherichia coli

Protein Variants

Protein Variants Comment Organism
D230N the variant has somewhat greater processivity than the wild-type enzyme Escherichia coli
E173K the variant has somewhat greater processivity than the wild-type enzyme Escherichia coli
E245K mutation leads to very high polyP accumulation in vivo but is not different from the wild type in either activity or chain length of polyP produced in vitro Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.06
-
 ATP pH 7.5, 37°C, mutant enzyme D230N Escherichia coli
2.07
-
 ATP pH 7.5, 37°C, wild-type enzyme Escherichia coli
2.08
-
 ATP pH 7.5, 37°C, mutant enzyme E245K Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (phosphate)n Escherichia coli
-
 ADP + (phosphate)n+1
-
 ?
ATP + (phosphate)n Escherichia coli K12
-
 ADP + (phosphate)n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7B1
-
-
Escherichia coli K12 P0A7B1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (phosphate)n
-
 Escherichia coli ADP + (phosphate)n+1
-
 ?
ATP + (phosphate)n
-
 Escherichia coli K12 ADP + (phosphate)n+1
-
 ?

Synonyms

Synonyms Comment Organism
polyP kinase
-
 Escherichia coli
PPK
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.135
-
 ADP pH 7.5, 37°C, mutant enzyme D230N Escherichia coli
0.144
-
 ADP pH 7.5, 37°C, mutant enzyme E245K Escherichia coli
0.156
-
 ADP pH 7.5, 37°C, wild-type enzyme Escherichia coli

General Information

General Information Comment Organism
malfunction a mutant lacking the enzyme (PPK) makes no detectable polyP under any condition Escherichia coli