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Literature summary for 2.7.4.1 extracted from

  • Batten, L.; Parnell, A.; Wells, N.; Murch, A.; Oyston, P.; Roach, P.
    Biochemical and structural characterization of polyphosphate kinase 2 from the intracellular pathogen Francisella tularensis (2016), Biosci. Rep., 36, e00294 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Francisella tularensis subsp. tularensis

Crystallization (Commentary)

Crystallization (Comment) Organism
structure to 2.23 resolution. The structure consists of a six-stranded parallel beta-sheet surrounded by 12 alpha-helices, with a high degree of similarity to other members of the PPK2 family Francisella tularensis subsp. tularensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.372
-
 ATP pH 8, 37°C Francisella tularensis subsp. tularensis
0.546
-
 ADP pH 8, 37°C Francisella tularensis subsp. tularensis

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum concentration 10 mM Francisella tularensis subsp. tularensis
Mn2+ optimum concentration 1 mM Francisella tularensis subsp. tularensis

Organism

Organism UniProt Comment Textmining
Francisella tularensis subsp. tularensis Q5NEQ5 cf. EC 2.7.4.34
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25
-
 synthesis of ATP, pH 8, 37°C Francisella tularensis subsp. tularensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)n+1
-
 Francisella tularensis subsp. tularensis ATP + (phosphate)n
-
 r
ATP + (phosphate)n
-
 Francisella tularensis subsp. tularensis ADP + (phosphate)n+1
-
 r
additional information enzyme also accepts GDP, reaction of Ec 2.7.4.34. No substrates: CDP, UDP Francisella tularensis subsp. tularensis ?
-
-

Synonyms

Synonyms Comment Organism
FTT_1564
-
 Francisella tularensis subsp. tularensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.46
-
 ATP pH 8, 37°C Francisella tularensis subsp. tularensis
3.17
-
 ADP pH 8, 37°C Francisella tularensis subsp. tularensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Francisella tularensis subsp. tularensis

General Information

General Information Comment Organism
physiological function a PPK2 mutant strain shows significantly increased sensitivity to a range of antibiotics in a manner independent of the mode of action of the antibiotic Francisella tularensis subsp. tularensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.92
-
 ATP pH 8, 37°C Francisella tularensis subsp. tularensis
5.788
-
 ADP pH 8, 37°C Francisella tularensis subsp. tularensis