Cloned (Comment) | Organism |
---|---|
gene ppk, subcloning in Escherichia coli strain TOP10 | Proteus mirabilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a ppk gene encoding the PPK insertional mutant in Proteus mirabilis strain HI4320 | Proteus mirabilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Proteus mirabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | Proteus mirabilis | - |
ADP + (phosphate)n+1 | - |
r | |
ATP + (phosphate)n | Proteus mirabilis HI4320 | - |
ADP + (phosphate)n+1 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus mirabilis | B4EY92 | gene ppk | - |
Proteus mirabilis HI4320 | B4EY92 | gene ppk | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | - |
Proteus mirabilis | ADP + (phosphate)n+1 | - |
r | |
ATP + (phosphate)n | - |
Proteus mirabilis HI4320 | ADP + (phosphate)n+1 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PPK | - |
Proteus mirabilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Proteus mirabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Proteus mirabilis |
General Information | Comment | Organism |
---|---|---|
malfunction | the ppk-deficient mutant is deficient in resistance to oxidative, hyperosmotic and heat stress. The swarming and biofilm formation abilities of Proteus mirabilis are also attenuated after the ppk interruption. Negative phenotypes of the ppk mutant can be restored by ppk gene complementation | Proteus mirabilis |
physiological function | enzyme PPK is an important regulator and plays a crucial role in stress tolerance and virulence in uropathogenic Proteus mirabilis, overview. Gene ppk is required for Proteus mirabilis to invade the bladder | Proteus mirabilis |