Activating Compound | Comment | Organism | Structure |
---|---|---|---|
basic protein | activation | Escherichia coli | |
bovine serum albumin | activation, can substitute for histone only in the absence of phosphate | Escherichia coli | |
casein | activation, can substitute for histone only in the absence of phosphate | Escherichia coli | |
histone | strong inhibition of reverse reaction | Escherichia coli | |
histone | maximum activation in the presence of 20 mM phosphate | Escherichia coli | |
phosphate | inhibiton above 20 mM | Escherichia coli | |
phosphate | required for activity, only in the presence of histone | Escherichia coli | |
protamine | required for maximal activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 0.2 mM, complete inhibition | Escherichia coli | |
diphosphate | 1 mM, complete inhibition | Escherichia coli | |
F- | 20 mM, complete inhibition | Escherichia coli | |
histone | reverse reaction, strong, activates forward reaction in the presence of phosphate | Escherichia coli | |
KCl | - |
Escherichia coli | |
additional information | not inhibited by 3',5'-adenosine monophosphate | Escherichia coli | |
NaCl | - |
Escherichia coli | |
phosphate | above 20 mM; activates in the presence of histone | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
ATP | pH 7.0, 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation | Escherichia coli | |
Mn2+ | activation, can replace Mg2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | Escherichia coli | involved in phosphate metabolism of bacteria | ADP + (phosphate)n+1 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain K-12, Crooke's strain | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, Sephadex G-200 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | - |
Escherichia coli | ADP + (phosphate)n+1 | - |
? | |
ATP + (phosphate)n | involved in phosphate metabolism of bacteria | Escherichia coli | ADP + (phosphate)n+1 | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Escherichia coli |