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Literature summary for 2.7.3.3 extracted from

  • Yano, D.; Suzuki, T.
    Kinetic analyses of the substrate inhibition of Paramecium arginine kinase (2018), Protein J., 37, 581-588 .
    View publication on PubMed
Search for more literature for 2.7.3.3

Protein Variants

Protein Variants Comment Organism
Q80A the SES complex dissociation constant (Ki SES: 0.64 mM) is two times larger than that of the wild-type as well as the ES complex dissociation constant (Ki ES: 0.29 mM). The regression curve for this mutant resembles that of the wild-type, suggesting that substrate inhibition is still present in this mutant Paramecium tetraurelia
S79A the SES complex dissociation constant (Ki SES: 3.62 mM) is ten times than that of the wild-type (0.34 mM). This suggests the significant involvement of the S79 residue in binding processes at the second arginine substrate, i.e., in displaying substrate inhibition Paramecium tetraurelia
V81A the SES complex dissociation constant (Ki SES: 2.16 mM) is significantly larger (seven times) than that of the wildtype, similar to the S79A mutant. The regression curve also resembles that of the S79A mutant, indicating that substrate inhibition is not absent, but rather weakened Paramecium tetraurelia

Inhibitors

Inhibitors Comment Organism Structure
L-arginine after primary arginine substrate binding (ES complex formation), the binding of another arginine at the secondarily induced inhibitory site is accelerated to form the SES complex, causing substrate inhibition. S79 and V81 in the Paramecium AK3 are the key residues involved in substrate inhibition Paramecium tetraurelia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-arginine Paramecium tetraurelia
-
 ADP + Nomega-phospho-L-arginine
-
 ?

Organism

Organism UniProt Comment Textmining
Paramecium tetraurelia
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine
-
 Paramecium tetraurelia ADP + Nomega-phospho-L-arginine
-
 ?

Synonyms

Synonyms Comment Organism
AK3
-
 Paramecium tetraurelia

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.73
-
 L-arginine 25°C, pH and temperature not specified in the publication, mutant enzyme S79A Paramecium tetraurelia
2.54
-
 L-arginine 25°C, pH and temperature not specified in the publication, mutant enzyme Q80A Paramecium tetraurelia
3.52
-
 L-arginine 25°C, pH and temperature not specified in the publication, wild-type enzyme Paramecium tetraurelia
5.45
-
 L-arginine 25°C, pH and temperature not specified in the publication, mutant enzyme V81A Paramecium tetraurelia