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Literature summary for 2.7.3.3 extracted from

  • Laino, A.; Lopez-Zavala, A.A.; Garcia-Orozco, K.D.; Carrasco-Miranda, J.S.; Santana, M.; Stojanoff, V.; Sotelo-Mundo, R.R.; Garcia, C.F.
    Biochemical and structural characterization of a novel arginine kinase from the spider Polybetes pythagoricus (2017), PeerJ, 5, e3787 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Rosetta Polybetes pythagoricus

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor-diffusion method, the apoenzyme and enzyme bound to arginine, both in the open conformation with the active site lid (residues 310-320) completely disordered. The guanidino group binding site in the apo structure appears to be organized to accept the arginine substrate Polybetes pythagoricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 L-arginine pH 8.6, 30°C Polybetes pythagoricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 immobilized metal ion affinity chromatography Polybetes pythagoricus

Organism

Organism UniProt Comment Textmining
Polybetes pythagoricus A0A286R7K5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Polybetes pythagoricus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Polybetes pythagoricus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + Nomega-phospho-L-arginine
-
 Polybetes pythagoricus ATP + L-arginine
-
 r
ATP + L-arginine
-
 Polybetes pythagoricus ADP + Nomega-phospho-L-arginine
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Polybetes pythagoricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
75
-
 L-arginine pH 8.6, 30°C Polybetes pythagoricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 assay at Polybetes pythagoricus

General Information

General Information Comment Organism
physiological function the enzyme is an enzyme crucial for energy metabolism, keeping the pool of phosphagens in invertebrates, and also an allergen for humans Polybetes pythagoricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
44.1
-
 L-arginine pH 8.6, 30°C Polybetes pythagoricus