Literature summary for 2.7.3.3 extracted from

Zhang, N.; Jiang, H.; Meng, X.; Qian, K.; Liu, Y.; Song, Q.; Stanley, D.; Wu, J.; Park, Y.; Wang, J.
Broad-complex transcription factor mediates opposing hormonal regulation of two phylogenetically distant arginine kinase genes in Tribolium castaneum (2020), Commun. Biol., 3, 631 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.4	-	Nomega-phospho-L-arginine	pH 7.5, 30°C, arginine kinase TcAK2	Tribolium castaneum
1.5	-	L-arginine	pH 7.5, 30°C, arginine kinase TcAK1	Tribolium castaneum
3.7	-	L-arginine	pH 7.5, 30°C, arginine kinase TcAK2	Tribolium castaneum
14	-	Nomega-phospho-L-arginine	pH 7.5, 30°C, arginine kinase TcAK1	Tribolium castaneum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	TcAK1 is detected in cytoplasm including mitochondria	Tribolium castaneum	5737	-
cytoplasm	TcAK2 is detected in cytoplasm excluding mitochondria	Tribolium castaneum	5737	-
mitochondrion	TcAK1 is detected in cytoplasm including mitochondria	Tribolium castaneum	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	SDS-PAGE	Tribolium castaneum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-arginine	Tribolium castaneum	-	ADP + Nomega-phospho-L-arginine	-	?

Organism

Organism	UniProt	Comment	Textmining
Tribolium castaneum	-	-	-
Tribolium castaneum	D6WJX7	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
egg	TcAK1 activity is the highest in 5-day-old larvae and lowest in 3-day-old eggs	Tribolium castaneum	-
fat body	lowest expression level of TcAK1 in midgut and fat body	Tribolium castaneum	-
fat body	lowest expression level of TcAK2 in midgut and fat body	Tribolium castaneum	-
head	highest expression level of TcAK1 gene in head followed by thorax	Tribolium castaneum	-
head	highest expression level of TcAK2 gene in head followed by thorax	Tribolium castaneum	-
larva	relatively stable arginine kinase (TcAK1) activity is observed during larval-pupal metamorphosis	Tribolium castaneum	-
larva	relatively stable arginine kinase (TcAK2) activity is observed during larval-pupal metamorphosis	Tribolium castaneum	-
midgut	lowest expression level of TcAK1 in midgut and fat body	Tribolium castaneum	-
midgut	lowest expression level of TcAK2 in midgut and fat body	Tribolium castaneum	-
pupa	relatively stable arginine kinase (TcAK1) activity is observed during larval-pupal metamorphosis	Tribolium castaneum	-
pupa	relatively stable arginine kinase (TcAK2) activity is observed during larval-pupal metamorphosis	Tribolium castaneum	-
thorax	highest expression level of TcAK1 gene in head followed by thorax	Tribolium castaneum	-
thorax	highest expression level of TcAK2 gene in head followed by thorax	Tribolium castaneum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + Nomega-phospho-L-arginine	-	Tribolium castaneum	ATP + L-arginine	-	r
ATP + L-arginine	-	Tribolium castaneum	ADP + Nomega-phospho-L-arginine	-	?
ATP + L-arginine	arginine kinase TcAK1 shows marked preference for L-arginine over D-arginine	Tribolium castaneum	ADP + Nomega-phospho-L-arginine	-	r
ATP + L-arginine	arginine kinase TcAK2 shows marked preferences for L-arginine over D-arginine. Arginine kinase TcAK2 shows no activity with L-Nalpha-acetylarginine and L-canavanine	Tribolium castaneum	ADP + Nomega-phospho-L-arginine	-	?
ATP + L-canavanine	arginine kinase TcAK1 shows approximately 15% reaction rate compared to L-arginine	Tribolium castaneum	ADP + ?	-	?
ATP + L-Nalpha-acetylarginine	arginine kinase TcAK1 shows approximately 15% reaction rate compared to L-arginine	Tribolium castaneum	ADP + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE	Tribolium castaneum

Synonyms

Synonyms	Comment	Organism
TcAK1	-	Tribolium castaneum
TcAK2	-	Tribolium castaneum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
12.7	-	L-arginine	pH 7.5, 30°C, arginine kinase TcAK2	Tribolium castaneum
22	-	Nomega-phospho-L-arginine	pH 7.5, 30°C, arginine kinase TcAK1	Tribolium castaneum
146.1	-	Nomega-phospho-L-arginine	pH 7.5, 30°C, arginine kinase TcAK2	Tribolium castaneum
172.7	-	L-arginine	pH 7.5, 30°C, arginine kinase TcAK1	Tribolium castaneum

Expression

Organism	Comment	Expression
Tribolium castaneum	TcAK1 expression is negatively regulated by 20-hydroxyecdysone	down
Tribolium castaneum	TcAK1 mRNA levels are significantly decreased by 1.42- and 2.31-fold in 1-day-old prepupa and 3-day-old prepupa, respectively	down
Tribolium castaneum	TcAK2 expression is negatively regulated by juvenile hormone	down
Tribolium castaneum	TcAK1 expression is positively regulated by juvenile hormone	up
Tribolium castaneum	TcAK2 expression is positively regulated by 20-hydroxyecdysone	up
Tribolium castaneum	the highest accumulation of mRNA encoding TcAK2 occurrs in 1-day-old males, with lower accumulations in the other stages. TcAK2 mRNA levels are sharply increased by 99fold and 593fold in 1-day-old prepupa and 3-day-old prepupa, respectively	up
Tribolium castaneum	the relative accumulation of mRNA encoding TcAK1 is the highest in 5-day-old larvae and lowest in 3-day-old eggs	up

General Information

General Information	Comment	Organism
metabolism	the opposite hormonal regulation of arginine kinase TcAK1 and arginine kinase TcAK2 is mediated by transcription factor Broad-Complex	Tribolium castaneum
physiological function	arginine kinase TcAK1 and arginine kinase TcAK2 play similar roles in adult fertility and stress response	Tribolium castaneum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.57	-	Nomega-phospho-L-arginine	pH 7.5, 30°C, arginine kinase TcAK1	Tribolium castaneum
3.5	-	L-arginine	pH 7.5, 30°C, arginine kinase TcAK2	Tribolium castaneum
104.36	-	Nomega-phospho-L-arginine	pH 7.5, 30°C, arginine kinase TcAK2	Tribolium castaneum
112.9	-	L-arginine	pH 7.5, 30°C, arginine kinase TcAK1	Tribolium castaneum

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Release 2023.1 (January 2023)