Cloned (Comment) | Organism |
---|---|
gene YH65_02995, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Sulfurovum lithotrophicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Sulfurovum lithotrophicum | |
0.071 | - |
ATP | pH 8.0, 30°C, recombinant wild-type enzyme | Sulfurovum lithotrophicum | |
0.401 | - |
L-arginine | pH 8.0, 25°C, recombinant wild-type enzyme | Sulfurovum lithotrophicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Sulfurovum lithotrophicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | Sulfurovum lithotrophicum | - |
ADP + Nomega-phospho-L-arginine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurovum lithotrophicum | - |
gene YH65_02995 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme in Escherichia coli strain BL21(DE3) by metal affinity chromatography | Sulfurovum lithotrophicum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-arginine = ADP + Nomega-phospho-L-arginine | random-order, rapid-equilibrium kinetic mechanism | Sulfurovum lithotrophicum |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | - |
Sulfurovum lithotrophicum | ADP + Nomega-phospho-L-arginine | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme sequence is structurally divided into two domains, N- and C-terminal domains | Sulfurovum lithotrophicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sulfurovum lithotrophicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
88 | - |
L-arginine | pH 8.0, 25°C, recombinant wild-type enzyme | Sulfurovum lithotrophicum | |
88 | - |
ATP | pH 8.0, 30°C, recombinant wild-type enzyme | Sulfurovum lithotrophicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sulfurovum lithotrophicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sulfurovum lithotrophicum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is widely distributed in invertebrate animals. The enzyme is also found in unicellular organisms, protists and bacteria, but its occurrence is intermittent among species. Detailed phylogenetic analysis, overview | Sulfurovum lithotrophicum |
physiological function | arginine kinase plays a key role in ATP buffering systems of tissues and nerves that display high and variable rates of ATP turnover | Sulfurovum lithotrophicum |