Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Lucilia cuprina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.594 | - |
L-Arg | native enzyme, in 50 mM TrisHCl pH 7.5, at 22°C | Lucilia cuprina | |
0.603 | - |
L-Arg | recombinant enzyme, in 50 mM TrisHCl pH 7.5, at 22°C | Lucilia cuprina | |
1.166 | - |
Nomega-phospho-L-Arg | native enzyme, in 50 mM TrisHCl pH 7.5, at 22°C | Lucilia cuprina | |
1.432 | - |
Nomega-phospho-L-Arg | recombinant enzyme, in 50 mM TrisHCl pH 7.5, at 22°C | Lucilia cuprina | |
2.796 | - |
L-arginine ethyl ester | recombinant enzyme, in 50 mM TrisHCl pH 7.5, at 22°C | Lucilia cuprina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Lucilia cuprina | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Lucilia cuprina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lucilia cuprina | H9BZ68 | Australian sheep blowfly | - |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography | Lucilia cuprina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
abdomen | - |
Lucilia cuprina | - |
head | - |
Lucilia cuprina | - |
thorax | the enzyme is more abundant in the thorax, where it represents up to 2% of the total soluble protein, compared to head or abdomen | Lucilia cuprina | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + Nomega-phospho-L-Arg | - |
Lucilia cuprina | ATP + L-Arg | - |
r | |
ATP + L-Arg | the enzyme is stereospecific for the L-form over the D-form of its specific substrate arginine | Lucilia cuprina | ADP + Nomega-phospho-L-Arg | - |
r | |
ATP + L-arginine ethyl ester | 6% activity compared to L-Arg | Lucilia cuprina | ADP + Nomega-phospho-L-Arg ethyl ester | - |
? | |
ATP + L-canavanine | about 16% activity compared to L-Arg | Lucilia cuprina | ? | - |
? | |
ATP + L-homoarginine | 7% activity compared to L-Arg | Lucilia cuprina | ADP + Nomega-phospho-L-homoarginine | - |
? | |
additional information | no activity with D-arginine, N-acetyl-L-arginine, agmatine, and creatine | Lucilia cuprina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Lucilia cuprina |
Synonyms | Comment | Organism |
---|---|---|
ATP:L-arginine omega-N-phosphotransferase | - |
Lucilia cuprina |