Cloned (Comment) | Organism |
---|---|
expressed as maltose-binding protein enzyme fusion protein in Escherichia coli TB-1 cells | Abyssogena kaikoi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Abyssogena kaikoi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abyssogena kaikoi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography | Abyssogena kaikoi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Abyssogena kaikoi | ADP + Nomega-phospho-L-Arg | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Abyssogena kaikoi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.73 | - |
L-Arg | two-domain enzyme, in 100 mM Tris-HCl (pH 8.0), at 10°C | Abyssogena kaikoi | |
4.3 | - |
L-Arg | two-domain enzyme, in 100 mM Tris-HCl (pH 8.0), at 25°C | Abyssogena kaikoi | |
7.4 | - |
L-Arg | isolated domain 2, in 100 mM Tris-HCl (pH 8.0), at 10°C | Abyssogena kaikoi | |
13.7 | - |
L-Arg | isolated domain 2, in 100 mM Tris-HCl (pH 8.0), at 25°C | Abyssogena kaikoi |