Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Codon Plus cells | Locusta migratoria manilensis |
Protein Variants | Comment | Organism |
---|---|---|
L113D | the mutant shows strongly decreased activity compared to the wild type enzyme | Locusta migratoria manilensis |
L113G | the mutant shows decreased activity compared to the wild type enzyme | Locusta migratoria manilensis |
L113I | the mutant shows about wild type Km and kcat values | Locusta migratoria manilensis |
L113K | the mutant shows strongly decreased activity (10.3% catalytic efficiency) compared to the wild type enzyme | Locusta migratoria manilensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.94 | - |
L-Arg | native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
0.951 | - |
L-Arg | recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
0.99 | - |
L-Arg | mutant enzyme L113I, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
1.27 | - |
ATP | recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
1.29 | - |
ATP | native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
1.42 | - |
ATP | mutant enzyme L113I, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
1.81 | - |
L-Arg | mutant enzyme L113G, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
2.42 | - |
ATP | mutant enzyme L113G, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
2.98 | - |
L-Arg | mutant enzyme L113D, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
3.25 | - |
L-Arg | mutant enzyme L113K, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
3.68 | - |
ATP | mutant enzyme L113D, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
4.15 | - |
ATP | mutant enzyme L113K, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Locusta migratoria manilensis | A6M9J4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Locusta migratoria manilensis | ADP + Nomega-phospho-L-Arg | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP: L-arginine phosphotransferase | - |
Locusta migratoria manilensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 65 | the wild type enzyme retains its activity well at temperatures lower than 48°C, and then its activity shows a steep decrease from 45 to 60°C and completely loses its activity at temperatures above 65°C | Locusta migratoria manilensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
56.21 | - |
L-Arg | mutant enzyme L113K, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
64.43 | - |
L-Arg | mutant enzyme L113D, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
105.6 | - |
L-Arg | mutant enzyme L113G, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
152.2 | - |
L-Arg | mutant enzyme L113I, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
159.4 | - |
L-Arg | recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
163 | - |
L-Arg | native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis |