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Literature summary for 2.7.3.3 extracted from

  • Tada, H.; Suzuki, T.
    Cooperativity in the two-domain arginine kinase from the sea anemone Anthopleura japonicus. II. Evidence from site-directed mutagenesis studies (2010), Int. J. Biol. Macromol., 47, 250-254.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Anthopleura japonica

Protein Variants

Protein Variants Comment Organism
additional information the kcat value of the mutant with six Lys residues in the linker region between domains D1 and D2 is reduced to 27.6% that of the two-domain wild-type enzyme Anthopleura japonica
Y68G the mutation in domain 1 or 2 leads to almost no catalytic activity Anthopleura japonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
1 * 40000, SDS-PAGE Anthopleura japonica

Organism

Organism UniProt Comment Textmining
Anthopleura japonica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Anthopleura japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
Anthopleura japonica ADP + Nomega-phospho-L-Arg
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Anthopleura japonica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
89
-
L-Arg mutant two-domain-enzyme D1(Y68G)-D2, in 100 mM Tris/HCl, pH 8.0 at 25°C Anthopleura japonica
104
-
L-Arg wild type one-domain-enzyme D1, in 100 mM Tris/HCl, pH 8.0 at 25°C Anthopleura japonica
123
-
L-Arg mutant two-domain-enzyme D1-D2(Y68G), in 100 mM Tris/HCl, pH 8.0 at 25°C Anthopleura japonica
157
-
L-Arg wild type one-domain-enzyme D2, in 100 mM Tris/HCl, pH 8.0 at 25°C Anthopleura japonica
187
-
L-Arg mutant two-domain-enzyme D1-Lys6-D2, in 100 mM Tris/HCl, pH 8.0 at 25°C Anthopleura japonica
678
-
L-Arg wild type two-domain-enzyme D1-D2, in 100 mM Tris/HCl, pH 8.0 at 25°C Anthopleura japonica