Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli by two prokaryotic expression plasmids, pET-30a and pET-28a28a. The recombinant protein is expressed as inclusion bodies using pET-30a. Using expression plasmid, pET-28a, and changing the expression conditions results in a soluble and functional form of arginine kinase | Locusta migratoria manilensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.951 | - |
L-arginine | - |
Locusta migratoria manilensis | |
1.27 | - |
ATP | - |
Locusta migratoria manilensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Locusta migratoria manilensis | A6M9J4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Locusta migratoria manilensis |
Renatured (Comment) | Organism |
---|---|
after denaturation, the recombinant AK is successfully renatured and confirmed to be enzymatically active. Addition of Tween-20 and SDS to the dilution system lead to higher renaturation efficiency | Locusta migratoria manilensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Locusta migratoria manilensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | - |
Locusta migratoria manilensis | ADP + Nomega-phospho-L-arginine | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
synthesis direction of phosphoarginine and ADP | Locusta migratoria manilensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 70 | 10°C: about 55% of maximal activity, 70°C: about 40% of maximal activity, synthesis direction of phosphoarginine and ADP | Locusta migratoria manilensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
159.4 | - |
ATP | - |
Locusta migratoria manilensis | |
159.4 | - |
L-arginine | - |
Locusta migratoria manilensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
synthesis direction of phosphoarginine and ADP | Locusta migratoria manilensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10.5 | pH 7.0: about 40% of maximal activity, pH 10.5: about 35% of maximal activity, synthesis direction of phosphoarginine and ADP | Locusta migratoria manilensis |