Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) | Apostichopus japonicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | 0.2 mM, about 90% loss of activity | Apostichopus japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
L-Arg | at 25°C | Apostichopus japonicus | |
1.01 | - |
ATP | at 25°C | Apostichopus japonicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required in complex with ATP | Apostichopus japonicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Apostichopus japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apostichopus japonicus | - |
sea cucumber | - |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography and Superdex 200HR 10/30 gel filtration | Apostichopus japonicus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Apostichopus japonicus | ADP + Nomega-phospho-L-Arg | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Apostichopus japonicus |
Synonyms | Comment | Organism |
---|---|---|
ATP:arginine N-phosphotransferase | - |
Apostichopus japonicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Apostichopus japonicus |