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Literature summary for 2.7.3.3 extracted from

  • Zhang, J.W.; Guo, Q.; Zhao, T.J.; Liu, T.T.; Wang, X.C.
    Two fused proteins combining Stichopus japonicus arginine kinase and rabbit muscle creatine kinase (2006), Biochemistry (Moscow), 71, 983-988.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) Apostichopus japonicus

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride 0.2 mM, about 90% loss of activity Apostichopus japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
L-Arg at 25°C Apostichopus japonicus
1.01
-
ATP at 25°C Apostichopus japonicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required in complex with ATP Apostichopus japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
2 * 42000, SDS-PAGE Apostichopus japonicus

Organism

Organism UniProt Comment Textmining
Apostichopus japonicus
-
sea cucumber
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography and Superdex 200HR 10/30 gel filtration Apostichopus japonicus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Arg
-
Apostichopus japonicus ADP + Nomega-phospho-L-Arg
-
r

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Apostichopus japonicus

Synonyms

Synonyms Comment Organism
ATP:arginine N-phosphotransferase
-
Apostichopus japonicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Apostichopus japonicus