Cloned (Comment) | Organism |
---|---|
domain 2 is separated from the two-domain enzyme and expressed in Escherichia coli, domain 2 still exhibits activity. Expression of mutants of domain 2 in Escherichia coli: H60G, H60R and D197G | Corbicula japonica |
Protein Variants | Comment | Organism |
---|---|---|
D197G | mutant of domain 2, affinity for Arg in mutant enzyme is reduced considerably, accompanied by a decrease in Vmax | Corbicula japonica |
H60G | mutant of domain 2, affinity for Arg in mutant enzyme is reduced considerably, accompanied by a decrease in Vmax | Corbicula japonica |
H60R | mutant of domain 2, affinity for Arg in mutant enzyme is reduced considerably, accompanied by a decrease in Vmax | Corbicula japonica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
L-Arg | 37°C, domain 2 | Corbicula japonica | |
0.42 | - |
L-Arg | 37°C, two-domain enzyme | Corbicula japonica | |
0.46 | - |
ATP | 37°C, two-domain enzyme | Corbicula japonica | |
0.52 | - |
L-Arg | 37°C, D197G mutant enzyme of domain 2 | Corbicula japonica | |
0.97 | - |
ATP | 37°C, domain 2 | Corbicula japonica | |
1.02 | - |
L-Arg | 37°C, H60R mutant of domain 2 | Corbicula japonica | |
3.6 | - |
L-Arg | 37°C, H60G mutant of domain 2 | Corbicula japonica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corbicula japonica | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Corbicula japonica | ADP + Nomega-phospho-L-Arg | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.92 | - |
ATP | 25°C, H60G mutant of domain 2 | Corbicula japonica | |
8.92 | - |
L-Arg | 25°C, H60G mutant of domain 2 | Corbicula japonica | |
9.53 | - |
ATP | 25°C, D197G mutant of domain 2 | Corbicula japonica | |
9.53 | - |
L-Arg | 25°C, D197G mutant of domain 2 | Corbicula japonica | |
18.1 | - |
ATP | 25°C, H60R mutant of domain 2 | Corbicula japonica | |
18.1 | - |
L-Arg | 25°C, H60R mutant of domain 2 | Corbicula japonica | |
44.7 | - |
ATP | 25°C, domain 2 | Corbicula japonica | |
44.7 | - |
L-Arg | 25°C, domain 2 | Corbicula japonica | |
73.3 | - |
ATP | 25°C, two-domain enzyme | Corbicula japonica | |
73.3 | - |
L-Arg | 25°C, two-domain enzyme | Corbicula japonica |