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Literature summary for 2.7.2.3 extracted from

  • Jin, C.; Zhu, X.; Wu, H.; Wang, Y.; Hu, X.
    Perturbation of phosphoglycerate kinase 1 (PGK1) only marginally affects glycolysis in cancer cells (2020), J. Biol. Chem., 295, 6425-6446 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00438
-
 3-phospho-D-glyceroyl phosphate at pH 7.4, temperature not specified in the publication Saccharomyces cerevisiae
0.00686
-
 3-phospho-D-glyceroyl phosphate at pH 7.4, temperature not specified in the publication Homo sapiens
0.146
-
 3-phospho-D-glycerate at pH 7.4, temperature not specified in the publication Saccharomyces cerevisiae
0.186
-
 3-phospho-D-glycerate at pH 7.4, temperature not specified in the publication Homo sapiens
0.221
-
 ADP at pH 7.4, temperature not specified in the publication Homo sapiens
0.27
-
 ADP at pH 7.4, temperature not specified in the publication Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM used in assay conditions Saccharomyces cerevisiae
Mg2+ 5 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + 3-phospho-D-glyceroyl phosphate Saccharomyces cerevisiae the enzyme favors the forward reaction ATP + 3-phospho-D-glycerate
-
 r
ADP + 3-phospho-D-glyceroyl phosphate Homo sapiens the enzyme favors the forward reaction ATP + 3-phospho-D-glycerate
-
 r
ATP + 3-phospho-D-glycerate Saccharomyces cerevisiae
-
 ADP + 3-phospho-D-glyceroyl phosphate
-
 r
ATP + 3-phospho-D-glycerate Homo sapiens
-
 ADP + 3-phospho-D-glyceroyl phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens P00558
-
-
Saccharomyces cerevisiae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
 Homo sapiens
-
cervical carcinoma cell
-
 Homo sapiens
-
colonic cancer cell
-
 Homo sapiens
-
gastric cancer cell
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
liver cancer cell
-
 Homo sapiens
-
lung cancer cell
-
 Homo sapiens
-
MGC-803 cell
-
 Homo sapiens
-
RKO cell
-
 Homo sapiens
-
SK-HEP-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + 3-phospho-D-glyceroyl phosphate the enzyme favors the forward reaction Saccharomyces cerevisiae ATP + 3-phospho-D-glycerate
-
 r
ADP + 3-phospho-D-glyceroyl phosphate the enzyme favors the forward reaction Homo sapiens ATP + 3-phospho-D-glycerate
-
 r
ATP + 3-phospho-D-glycerate
-
 Saccharomyces cerevisiae ADP + 3-phospho-D-glyceroyl phosphate
-
 r
ATP + 3-phospho-D-glycerate
-
 Homo sapiens ADP + 3-phospho-D-glyceroyl phosphate
-
 r

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Saccharomyces cerevisiae
? x * 40000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
PGK1
-
 Saccharomyces cerevisiae
PGK1
-
 Homo sapiens
phosphoglycerate kinase 1
-
 Saccharomyces cerevisiae
phosphoglycerate kinase 1
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction perturbation of enzyme form PGK1 in cancer cells insignificantly affects the conversion of glucose to lactate in glycolysis. Enzyme knockdown does not significantly affect the glycolysis rate, moderately reduces serine consumption and de novo serine synthesis Homo sapiens