Literature summary for 2.7.2.3 extracted from

Mandelman, D.; Ballut, L.; Wolff, D.A.; Feller, G.; Gerday, C.; Haser, R.; Aghajari, N.
Structural determinants increasing flexibility confer cold adaptation in psychrophilic phosphoglycerate kinase (2019), Extremophiles, 23, 495-506 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas sp. TACII 18

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.2 M MgCl2 and 0.1 M Tris-HCl at pH 8.4	Pseudomonas sp. TACII 18

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP + 1,3-bisphosphoglycerate	Pseudomonas sp. TACII 18	-	ATP + 3-phosphoglycerate	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. TACII 18	Q9RBS3	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Ni-NTA column chromatography and Superdex S200 gel filtration	Pseudomonas sp. TACII 18

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + 1,3-bisphosphoglycerate	-	Pseudomonas sp. TACII 18	ATP + 3-phosphoglycerate	-	r

Synonyms

Synonyms	Comment	Organism
PGK	-	Pseudomonas sp. TACII 18

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Release 2023.1 (January 2023)