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Literature summary for 2.7.2.3 extracted from
- Molecular and biochemical characterization of natural and recombinant phosphoglycerate kinase B from Trypanosoma rangeli (2018), Exp. Parasitol., 187, 42-48 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta cells | Trypanosoma rangeli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
ATP | natural enzyme, at pH 7.6 and 25°C | Trypanosoma rangeli |  |
| 0.28 | - |
3-phospho-D-glycerate | natural enzyme, at pH 7.6 and 25°C | Trypanosoma rangeli | |
| 0.5 | - |
ATP | recombinant enzyme, at pH 7.6 and 25°C | Trypanosoma rangeli | |
| 0.71 | - |
3-phospho-D-glycerate | recombinant enzyme, at pH 7.6 and 25°C | Trypanosoma rangeli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Trypanosoma rangeli | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2 mM used in assay conditions | Trypanosoma rangeli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + 3-phospho-D-glyceroyl phosphate | Trypanosoma rangeli | - |
ATP + 3-phospho-D-glycerate | - |
r | |
| ADP + 3-phospho-D-glyceroyl phosphate | Trypanosoma rangeli SC58 | - |
ATP + 3-phospho-D-glycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | Trypanosoma rangeli | - |
ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
| ATP + 3-phospho-D-glycerate | Trypanosoma rangeli SC58 | - |
ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma rangeli | A0A061J5A1 | - |
- |
| Trypanosoma rangeli SC58 | A0A061J5A1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q Sepharose chromatography (natural enzyme) or ammonium sulfate precipitation, HiPrep Q column chromatography and Superdex 200 gel filtration (recombinant enzyme) | Trypanosoma rangeli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epimastigote | - |
Trypanosoma rangeli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + 3-phospho-D-glyceroyl phosphate | - |
Trypanosoma rangeli | ATP + 3-phospho-D-glycerate | - |
r | |
| ADP + 3-phospho-D-glyceroyl phosphate | - |
Trypanosoma rangeli SC58 | ATP + 3-phospho-D-glycerate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Trypanosoma rangeli | ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
| ATP + 3-phospho-D-glycerate | - |
Trypanosoma rangeli SC58 | ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 44000, SDS-PAGE | Trypanosoma rangeli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PGKB | - |
Trypanosoma rangeli |
| phosphoglycerate kinase B | - |
Trypanosoma rangeli |
| TRSC58_02767 | - |
Trypanosoma rangeli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Trypanosoma rangeli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | more than 70% activity between pH 8.0 and 10.0 | Trypanosoma rangeli |
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