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Literature summary for 2.7.2.18 extracted from

  • Harwood, C.S.; Canale-Parola, E.
    Properties of acetate kinase isozymes and a branched-chain fatty acid kinase from a spirochete (1982), J. Bacteriol., 152, 246-254 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.18

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ complete inhibition at 4 mM Ca2+ Spirochaetales
Co2+ 65% residual activity in the presence of 4 mM Spirochaetales
Cu2+ 58% residual activity in the presence of 4 mM Spirochaetales
K+ complete inhibition at 4 mM K+ Spirochaetales
Na+ complete inhibition at 4 mM Na+ Spirochaetales
Zn2+ 42% residual activity in the presence of 4 mM Spirochaetales

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 ATP with isobutyrate as cosubstrate, at pH 7.5 and 30°C Spirochaetales
4.3
-
 Isobutyrate at pH 7.5 and 30°C Spirochaetales
9.5
-
 Isovalerate at pH 7.5 and 30°C Spirochaetales
10.8
-
 2-methylbutyrate at pH 7.5 and 30°C Spirochaetales
12.5
-
 valerate at pH 7.5 and 30°C Spirochaetales
14.3
-
 propionate at pH 7.5 and 30°C Spirochaetales
16.9
-
 Butyrate at pH 7.5 and 30°C Spirochaetales

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 96% activity in the presence of 4 mM Mg2+ as compared to Mn2+ Spirochaetales
Mn2+ 100% activity in the presence of 4 mM Mn2+ Spirochaetales

Organism

Organism UniProt Comment Textmining
Spirochaetales
-
 spirochete MA-2
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography and Sephacryl S-300 gel filtration Spirochaetales

Storage Stability

Storage Stability Organism
-50°C, crude cell extract, 2 months, no loss of activity Spirochaetales
5°C, purified enzyme, 48 h, no loss of activity Spirochaetales

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methylbutyrate
-
 Spirochaetales ADP + 2-methylbutyryl phosphate
-
 ?
ATP + butyrate
-
 Spirochaetales ADP + butyryl phosphate
-
 ?
ATP + isobutyrate 100% activity Spirochaetales ADP + isobutyryl phosphate
-
 ?
ATP + isovalerate
-
 Spirochaetales ADP + isovaleryl phosphate
-
 ?
ATP + propionate
-
 Spirochaetales ADP + propionyl phosphate
-
 ?
ATP + valerate
-
 Spirochaetales ADP + valeryl phosphate
-
 ?
CTP + isobutyrate 103% activity compared to ATP Spirochaetales CDP + isobutyryl phosphate
-
 ?
GTP + isobutyrate 70% activity compared to ATP Spirochaetales GDP + isobutyryl phosphate
-
 ?
ITP + isobutyrate 68% activity compared to ATP Spirochaetales IDP + isobutyryl phosphate
-
 ?
additional information no activity with acetate Spirochaetales ?
-
-
UTP + isobutyrate 78% activity compared to ATP Spirochaetales UDP + isobutyryl phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE Spirochaetales

Synonyms

Synonyms Comment Organism
branched-chain fatty acid kinase
-
 Spirochaetales

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 60 25% loss of activity after 30 min at 45°C, complete loss of activity after 30 min at 60°C Spirochaetales

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Spirochaetales

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 more than 80% activity between pH 6.0 and 8.5 Spirochaetales

General Information

General Information Comment Organism
metabolism the enzyme is specific for leucine, isoleucine, and valine fermentation Spirochaetales