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Literature summary for 2.7.2.12 extracted from

  • Pineda, E.; Vazquez, C.; Encalada, R.; Nozaki, T.; Sato, E.; Hanadate, Y.; Nequiz, M.; Olivos-Garcia, A.; Moreno-Sanchez, R.; Saavedra, E.
    Roles of acetyl-CoA synthetase (ADP-forming) and acetate kinase (PPi-forming) in ATP and PPi supply in Entamoeba histolytica (2016), Biochim. Biophys. Acta, 1860, 1163-1172 .
    View publication on PubMed
Search for more literature for 2.7.2.12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 acetyl phosphate at pH 6.0 and 37°C Entamoeba histolytica
0.07
-
 acetyl phosphate at pH 6.0 and 37°C Entamoeba histolytica
2.2
-
 phosphate at pH 6.0 and 37°C Entamoeba histolytica
2.6
-
 phosphate at pH 6.0 and 37°C Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica C4M1C3
-
-

Purification (Commentary)

Purification (Comment) Organism
Co2+ affinity column chromatography Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information D-glucose 6-phosphate, D-fructose 6-phosphate, D-fructose 1,6-bisphophate, ADP, ATP and AMP used at the physiological concentrations and 1 mM each of 3-phosphoglycerate, 2-phosphoglycerate, phosphoenolpyruvate, UDP-glucose, UTP, O-phosphoserine and phosphocreatine are no substrates for the enzyme. The enzyme has no activity in the acetyl phosphate-forming direction Entamoeba histolytica ?
-
-
phosphate + acetyl phosphate
-
 Entamoeba histolytica diphosphate + acetate
-
 ir

Subunits

Subunits Comment Organism
homodimer 2 * 45000, His-tagged enzyme, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
acetate kinase (PPi-forming)
-
 Entamoeba histolytica
ACK
-
 Entamoeba histolytica
PPi-forming acetate kinase
-
 Entamoeba histolytica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
 phosphate at pH 6.0 and 37°C Entamoeba histolytica
21
-
 acetyl phosphate at pH 6.0 and 37°C Entamoeba histolytica
30
-
 phosphate at pH 7.0 and 37°C Entamoeba histolytica
30
-
 acetyl phosphate at pH 7.0 and 37°C Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Entamoeba histolytica

General Information

General Information Comment Organism
metabolism the enzyme does not contribute to acetate production but might be marginally involved in diphosphate and hexosephosphate homeostasis Entamoeba histolytica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9
-
 phosphate at pH 6.0 and 37°C Entamoeba histolytica
12
-
 phosphate at pH 6.0 and 37°C Entamoeba histolytica
355
-
 acetyl phosphate at pH 6.0 and 37°C Entamoeba histolytica
1400
-
 acetyl phosphate at pH 6.0 and 37°C Entamoeba histolytica