Cloned (Comment) | Organism |
---|---|
expression of Myc-tagged wild-type and mutant GRK2s in HEK-293T cells, co-expression with M33 in wild-type and Galphaq/11-/- mouse embryonic fibroblasts, M33 couples directly to the Gq/11 signaling pathway to induce high levels of total inositol phosphates in an agonist-independent manner | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D110A | the point mutation within the RH domain abrogates GRK2 sequestration of activated GTP-boundGalphaq/11 | Mus musculus |
D110A/K220R | the mutant, which exhibits no RH or kinase activity, is completely defective in its ability to attenuate M33 signaling | Mus musculus |
K220R | the point mutation within the kinase domain inhibits GRK2 catalytic activity | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein M33 | Mus musculus | GRK2 is a potent regulator of the mouse cytomegalovirus GPCR protein M33-induced Gq/11 signaling through its ability to phosphorylate M33 and sequester Galphaq/11 proteins dependent on an intact RH domain, the protein M33 is able to induce inositol phosphate accumulation, activate NF-kappaB, and promote smooth muscle cell migration, viral GPCRs like M33 play a role in viral dissemination in vivo, M33 is required for efficient murine cytomegalovirus replication in the mouse, and induces several signlaing pathways, overview | ADP + phosphorylated protein M33 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein M33 | GRK2 is a potent regulator of the mouse cytomegalovirus GPCR protein M33-induced Gq/11 signaling through its ability to phosphorylate M33 and sequester Galphaq/11 proteins dependent on an intact RH domain, the protein M33 is able to induce inositol phosphate accumulation, activate NF-kappaB, and promote smooth muscle cell migration, viral GPCRs like M33 play a role in viral dissemination in vivo, M33 is required for efficient murine cytomegalovirus replication in the mouse, and induces several signlaing pathways, overview | Mus musculus | ADP + phosphorylated protein M33 | - |
? | |
ATP + protein M33 | recombinant FLAG-tagged M33 expressed in HEK-293T cells | Mus musculus | ADP + phosphorylated protein M33 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G protein-coupled receptor kinase 2 | - |
Mus musculus |
GPCR kinase 2 | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |