Activating Compound | Comment | Organism | Structure |
---|---|---|---|
G protein betagamma-subunit | from bovine brain, beta-ARK 1 and 2: requirement, selectivity for betagamma subunits, both isoforms differentiate between defined betagamma subunits | Bos taurus |
Cloned (Comment) | Organism |
---|---|
beta-ARK 1 and 2 are cloned and expressed in SF9 cells using the baculovirus expression system | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
propranolol | beta-adrenergic antagonist, in presence no phosphorylation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
enzyme exists in 2 subtypes: beta-ARK 1 and beta-ARK 2 | - |
Bos taurus | - |
beta-ARK 1 and 2 | - |
Purification (Comment) | Organism |
---|---|
recombinant beta-ARK 1 and 2 overexpressed in Sf9 cells | Bos taurus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
beta-ARK 2 has a 25% lower specific activity than beta-ARK 1 towards rhodopsin and beta2-AR | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-adrenergic receptor | beta2-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | beta-ARK 2 has a 25% lower specific activity than beta-ARK 1 towards rhodopsin and beta2-AR | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + rhodopsin | beta-ARK 2 has a 25% lower specific activity than beta-ARK 1 towards rhodopsin and beta2-AR | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | in form of bovine rod outer segments | Bos taurus | ADP + phosphorhodopsin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bos taurus | beta-ARK 2 has a higher isoelectric point than beta-ARK 1 | - |
additional information |