Cloned (Comment) | Organism |
---|---|
recombinant CK1 undergoes autoinactivation via autophosphorylation in Escherichia coli cells and thus is undesirably prepared as a phosphorylated and inactivated kinase. To circumvent this problem, a protein expression system is established using Escherichia coli strain BL21(DE3)plambdaPP in which lambda protein phosphatase (lambdaPPase) is constitutively expressed. Using this system, recombinant CK1 isoforms (alpha, delta and epsilon) are readily prepared as unphosphorylated forms. CK1s prepared using BL21(DE3)plambdaPP show markedly higher activity than those prepared by the conventional BL21(DE3). The kinase activity of CK1delta from BL21(DE3)plambdaPP is higher than that prepared by a conventional method consisting of troublesome steps such as in vitro lambdaPPase treatment. The simple method using BL21(DE3)plambdaPP is valuable for preparing highly active CK1s | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8BK63 | - |
- |
Mus musculus | Q9DC28 | - |
- |
Mus musculus | Q9JMK2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
Synonyms | Comment | Organism |
---|---|---|
casein kinase 1 | - |
Mus musculus |
CK1alpha | - |
Mus musculus |
CK1delta | - |
Mus musculus |
CK1epsilon | - |
Mus musculus |