Activating Compound | Comment | Organism | Structure |
---|---|---|---|
H2O2 | - |
Homo sapiens | |
additional information | STK38 activity is stimulated by oxidative stresses such as hydrogen peroxide and X-ray irradiation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene STK38, recombinant expression in HeLa cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme silencing by expression of short hairpin (sh) RNA targeting STK38, or negative control shRNA, in LU-99 cells via transfection by electroporation | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CDC25A | Homo sapiens | STK38 phosphorylates CDC25A at Ser76 | ADP + phospho-CDC25A | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q15208 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
Lu-99 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CDC25A | STK38 phosphorylates CDC25A at Ser76 | Homo sapiens | ADP + phospho-CDC25A | - |
? | |
ATP + CDC25A | recombinantly expressed full-length wild-type CDC25A or CDC25A mutants S124A and S76A are used as substrates. STK38 phosphorylates wild-type CDC25A and mutant S124A at Ser76, it shows no activity with the S76A mutant | Homo sapiens | ADP + phospho-CDC25A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NDR1 | - |
Homo sapiens |
serine-threonine kinase 38 | - |
Homo sapiens |
STK38 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | STK38 is a member of the NDR family, which functions in cell-cyclerelated processes | Homo sapiens |
malfunction | the cellular depletion of STK38 has no effect on the G1-phase transition under normal conditions, but leads to a slight increase in G1 arrest after X-ray irradiation. The depletion of STK38 alone may be insufficient to activate the G1 checkpoint. STK38 depletion has no effect on the distribution of cells in S-phase under normal conditions | Homo sapiens |
physiological function | serine-threonine kinase 38 regulates CDC25A stability and the DNA damage-induced G2/M checkpoint. Enzyme STK38 is required for CDC25A degradation. Phosphorylation of Ser76 by STK38 regulates CDC25A's stability. Wild-type CDC25A is rapidly degraded, whereas the S76A mutant remains stable. STK38 is not involved in regulating the S-phase checkpoint, but enzyme CHK1 regulates the S-phase checkpoint by phosphorylating CDC25A at a number of sites, including Ser124. While CHK1 phosphorylates CDC25A at multiple sites, STK38's phosphorylation of CD25A may be more limited | Homo sapiens |