Cloned (Comment) | Organism |
---|---|
feline sarcoma oncogene, c-fps/fes, located at chromosome 15q25-qter, expression of a 281 amino acid sequence fragment as His-Fes-CR-FLAG in Escherichia coli strain M15pREP4 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His- and FLAG-tagged catalytic domain of Fes, hanging drop vapour diffusion method, 0.002 ml of 10 mg/ml protein solution, phosphate buffered saline, pH 7.4, is mixed with 0.002 ml reservoir solution and equilibrated against 0.4 ml reservoir solution containing 1.6 M magnesium sulfate and 0.1 M MES pH 6.5, 27°C, X-ray diffraction structure determination and analysis at 3.9 A resolution | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | Fes is involved in the regulation of cell-cell and cell-matrix interactions mediated by adherens junctions and focal adhesions, the activated form of the kinase can induce cellular transformation, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07332 | gene c-fps/fes, the feline sarcoma oncogene | - |
Purification (Comment) | Organism |
---|---|
recombinant His- and FLAG-tagged catalytic domain of Fes from Escherichia coli strain M15 by nickel affinity chromatography and diaflitration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
marrow cell | - |
Homo sapiens | - |
neuron | - |
Homo sapiens | - |
vascular endothelial cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Fes is involved in the regulation of cell-cell and cell-matrix interactions mediated by adherens junctions and focal adhesions, the activated form of the kinase can induce cellular transformation, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the N-terminus of Fes contains the FCH, i.e. Fps/Fes/Fer/CIP4 homology, domain, which is unique to the Fes/Fer kinase family, followed by three coiled-coil domains and an SH2, i.e. Src-homology 2, domain, the catalytic region Fes-CR is located at the C-terminus of the protein | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the nonreceptor protein-tyrosine kinase, PTK, family | Homo sapiens |
nonreceptor tyrosine kinase Fes | - |
Homo sapiens |