Cloned (Comment) | Organism |
---|---|
expression of wild-type and N114A mutant SH3 domain in Escherichia coli strain BL21 (DE3) | Abelson leukaemia virus |
Crystallization (Comment) | Organism |
---|---|
wild-type and mutant Abl tyrosine kinase SH3 domains complexed with the high-affinity peptide p41 APSYSPPPPP, 8 mg/ml protein in 50 mM glycine buffer, pH 3.0, mixed in a molar protein:ligand ratio of 1:2 with lyophilized p41, method development with different precipitant solutions, optimally at pH 7.0, X-ray diffraction structure determination and analysis at 1.75 A resolution, molecular replacement | Abelson leukaemia virus |
Protein Variants | Comment | Organism |
---|---|---|
N114A | site-directed mutagenesis in the proline-rich region, mutant of the SH3 domain, structure determination in a complex with a proline-rich peptide, comparison to the wild-type enzyme, overview | Abelson leukaemia virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics | Abelson leukaemia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abelson leukaemia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and N114A mutant SH3 domain from Escherichia coli strain BL21 (DE3) by ammonium sulfate fractionation and gel filtration | Abelson leukaemia virus |
Synonyms | Comment | Organism |
---|---|---|
Abl tyrosine kinase | - |
Abelson leukaemia virus |