Cloned (Comment) | Organism |
---|---|
expression of Chk as soluble dual affinity tag fusion protein, with a GST-tag at the N-terminus and a Strep-tag at the C-terminus, in Escherichia coli strain DH5alpha | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-amino-5-(4-chlorophenyl)-7-(tert-butyl)pyrazolo[3,4-d] pyrimidine | i.e. pp2, inhibition of Csk involves Thr265, no inhibition of Chk | Homo sapiens | |
Mn2+ | inhibitory above 1 mM | Homo sapiens | |
additional information | enzyme is cleaved by several proteases such as thrombin | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
poly(Glu4-Tyr) | recombinant GST-fusion Chk, 12 mM Mg2+, no Mn2+ | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Chk requires about 10 mM Mg2+ for full activity | Homo sapiens | |
Mn2+ | optimal at 0.5 mM, inhibitory above 1 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 77000, recombinant GST-fusion Chk, SDS-PAGE, x * 50000, proteolytic fragment of recombinant GST-fusion Chk, SDS-PAGE | Homo sapiens |
77000 | - |
x * 77000, recombinant GST-fusion Chk, SDS-PAGE, x * 50000, proteolytic fragment of recombinant GST-fusion Chk, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Strep-tagged GST-fusion Chk from Escherichia coli strain DH5alpha 6fold by glutathione and streptavidin affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0173 | - |
purified recombinant Strep-tagged GST-fusion Chk | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + lysozyme-L-tyrosine | substrate of Src, low activity with Csk or Chk | Homo sapiens | ADP + lysozyme-L-tyrosine phosphate | - |
? | |
ATP + poly(Glu4-Tyr) | - |
Homo sapiens | ADP + poly(Glu4-Tyr)-L-tyrosine phosphate | - |
? | |
ATP + [kdSrc kinase]-L-tyrosine | i.e. kinase-defective Src, substrate of Chk and Csk, inactivation mechanism of Src, overview | Homo sapiens | ADP + [kdSrc kinase]-L-tyrosine phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77000, recombinant GST-fusion Chk, SDS-PAGE, x * 50000, proteolytic fragment of recombinant GST-fusion Chk, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CHK | - |
Homo sapiens |
Csk | - |
Homo sapiens |
More | Chk belongs to the Src family of PTKs | Homo sapiens |
Protein tyrosine kinase | - |
Homo sapiens |
PTK | - |
Homo sapiens |
Src | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |