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Literature summary for 2.7.1.95 extracted from

  • Numata, K.; Horii, Y.; Motoda, Y.; Hirai, N.; Nishitani, C.; Watanabe, S.; Kigawa, T.; Kodama, Y.
    Direct introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance (2016), Plant Biotechnol., 33, 403-407 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.95

Cloned(Commentary)

Cloned (Comment) Organism
expressed in leaves of JM1 apple (Malus prunifolia cultivar Seishi x Malus pumila paradisiaca cultivar M.9) Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + kanamycin Escherichia coli
-
 ADP + kanamycin 3'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + kanamycin
-
 Escherichia coli ADP + kanamycin 3'-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 32300, His-tagged enzyme, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
neomycin phosphotransferase II
-
 Escherichia coli
NPTII
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance Escherichia coli