Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | weak | Entamoeba histolytica | |
Ca2+ | - |
Entamoeba histolytica | |
D-fructose 6-phosphate | competitive versus D-fructose 1,6-bisphosphate and phosphate | Entamoeba histolytica | |
diphosphate | - |
Entamoeba histolytica | |
EDTA | - |
Entamoeba histolytica | |
Ni2+ | - |
Entamoeba histolytica | |
phosphate | competitive versus diphosphate | Entamoeba histolytica | |
Zn2+ | - |
Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
diphosphate | pH 7.0 | Entamoeba histolytica | |
0.008 | - |
Mg2+ | forward reaction, pH 7.0, 30°C | Entamoeba histolytica | |
0.009 | - |
D-fructose 1,6-bisphosphate | pH 7.0, 30°C | Entamoeba histolytica | |
0.5 | - |
Mg2+ | reverse reaction, pH 7.0, 30°C | Entamoeba histolytica | |
0.59 | 1 | phosphate | - |
Entamoeba histolytica | |
3.6 | - |
arsenate | pH 7.0, 30°C | Entamoeba histolytica | |
3.6 | - |
arsenate | with D-fructose 1,6-bisphosphate | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | less effective than Mg2+ | Entamoeba histolytica | |
Co2+ | less effective than Mn2+ | Entamoeba histolytica | |
divalent cations | required | Entamoeba histolytica | |
divalent cations | desecending order Mn2+, Mg2+, Co2+ | Entamoeba histolytica | |
Mg2+ | requirement | Entamoeba histolytica | |
Mg2+ | less effective than Mn2+ | Entamoeba histolytica | |
Mg2+ | enzyme requires 0.5 mM in the forward reaction, 0.0008 mM in the reverse reaction | Entamoeba histolytica | |
Mn2+ | requirement | Entamoeba histolytica | |
additional information | no activation by Ni2+ | Entamoeba histolytica | |
additional information | no activation by Zn2+ | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
gel filtration | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | Entamoeba histolytica | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
diphosphate + D-fructose 6-phosphate | Entamoeba histolytica DKB | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
DKB-strain | - |
Entamoeba histolytica DKB | - |
DKB-strain | - |
Purification (Comment) | Organism |
---|---|
partial | Entamoeba histolytica |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate | random bi bi mechanism | Entamoeba histolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
partially purified enzyme | Entamoeba histolytica |
Storage Stability | Organism |
---|---|
-20°C, partially purified enzyme, loss of 25% activity after 1 month | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arsenate + D-fructose 1,6-bisphosphate | - |
Entamoeba histolytica | ? | - |
r | |
arsenate + D-fructose 1,6-bisphosphate | - |
Entamoeba histolytica DKB | ? | - |
r | |
diphosphate + D-fructose 6-phosphate | - |
Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
diphosphate + D-fructose 6-phosphate | no phosphate group exchange reaction between phosphate and diphosphate, or D-fructose 6-phosphate and D-fructose 1,6-bisphosphate in absence of a third substrate | Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
diphosphate + D-fructose 6-phosphate | - |
Entamoeba histolytica DKB | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
diphosphate + D-fructose 6-phosphate | no phosphate group exchange reaction between phosphate and diphosphate, or D-fructose 6-phosphate and D-fructose 1,6-bisphosphate in absence of a third substrate | Entamoeba histolytica DKB | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Entamoeba histolytica DKB | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.115 | - |
D-fructose 6-phosphate | versus diphosphate, pH 7.0, 30°C | Entamoeba histolytica | |
0.16 | - |
D-fructose 6-phosphate | versus D-fructose 1,6-bisphosphate, pH 7.0, 30°C | Entamoeba histolytica | |
0.85 | - |
phosphate | versus diphosphate, pH 7.0, 30°C | Entamoeba histolytica | |
3 | - |
phosphate | versus D-fructose 1,6-bisphosphate, pH 7.0, 30°C | Entamoeba histolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Entamoeba histolytica | - |
- |
5.8 |