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Literature summary for 2.7.1.86 extracted from

  • Tomas-Gamisans, M.; Andrade, C.C.P.; Maresca, F.; Monforte, S.; Ferrer, P.; Albiol, J.
    Redox engineering by ectopic overexpression of NADH kinase in recombinant Pichia pastoris (Komagataella phaffii) impact on cell physiology and recombinant production of secreted proteins (2020), Appl. Environ. Microbiol., 86, e02038-19 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
biotechnology recombinant protein overproduction often results in oxidative stress, causing deviations from the optimal redox cofactor regeneration balance. This becomes one of the limiting factors in obtaining high levels of heterologous protein production. Overexpression of NADH kinase enzyme from Saccharomyces cerevisiae (Pos5) in the cytosol of Pichia pastoris results in a significant improvement in the production of the model protein Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
overpressed in Pichia pastoris, either directed to the cytosol or to the mitochondria. POS5 overexpression increases the NADPH/NADPx02 ratio in all the strains and under all conditions tested Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Synonyms

Synonyms Comment Organism
Pos5
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 Saccharomyces cerevisiae