Inhibitors | Comment | Organism | Structure |
---|---|---|---|
choline | ethanolamine kinase I not inhibited, ethanolamine kinase II strongly inhibited | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can substitute for Mg2+ | Rattus norvegicus | |
Mg2+ | ethanolamine kinase I: uses MgATP2- as substrate but also has an additional requirement for Mg2+ | Rattus norvegicus | |
Mg2+ | maximal activation at 16 mM MgATP2-, inhibition above | Rattus norvegicus | |
Mg2+ | Km MgATP2-: 14 mM | Rattus norvegicus | |
Mg2+ | ethanolamine kinase II: requires MgATP2- as substrate, maximal activation with 10 mM of Mg2+ and ATP | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
ethanolamine kinase I, gel filtration | Rattus norvegicus |
160000 | - |
ethanolamine kinase II, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ethanolamine kinase I and II | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + ethanolamine = ADP + O-phosphoethanolamine | ethanolamine kinase II and choline kinase do not use a common active site | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-20°C, 0.02 M phosphate buffer, pH 7.0, 2.9 mM DTT, protein concentration 1.0 mg/ml, ethanolamine kinase I, 2 days, 40% loss of activity | Rattus norvegicus |
4°C, 1 week, ethanolamine kinase I, 50% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ethanolamine | - |
Rattus norvegicus | ADP + O-phosphoethanolamine | - |
? | |
choline + ATP | ethanolamine kinase II, not ethanolamine kinase I | Rattus norvegicus | ADP + O-phosphocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 2.7.1.32 | Rattus norvegicus |
More | ethanolamine kinase II and choline kinase do not use a common active site | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | ethanolamine kinase I | Rattus norvegicus |
8 | 9.5 | ethanolamine kinase II | Rattus norvegicus |