BRENDA - Enzyme Database show
show all sequences of 2.7.1.76

Deoxynucleoside kinases encoded by the yaaG and yaaF genes of Bacillus subtilis. Substrate specificity and kinetic analysis of deoxyguanosine kinase with UTP as the preferred phosphate donor

Andersen, R.B.; Neuhard, J.; J. Biol. Chem. 276, 5518-5524 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene yaaF of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required for activity
Bacillus subtilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
gene yaaF, yaaF encodes an enzyme with specificity for deoxyadenosine and deoxycytidine
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3
-
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + 2'-deoxyadenosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
GTP + 2'-deoxycytidine
-
640561
Bacillus subtilis
GDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
additional information
substrate specificity, enzyme is specific for deoxyadenosine and deoxycytidine
640561
Bacillus subtilis
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
GTP
-
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene yaaF of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
GTP
-
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required for activity
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3
-
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + 2'-deoxyadenosine
-
640561
Bacillus subtilis
GDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
GTP + 2'-deoxycytidine
-
640561
Bacillus subtilis
GDP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
additional information
substrate specificity, enzyme is specific for deoxyadenosine and deoxycytidine
640561
Bacillus subtilis
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Bacillus subtilis
Other publictions for EC 2.7.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739204
Clausen
Plants salvage deoxyribonucleo ...
Arabidopsis thaliana, Solanum tuberosum
Nucleosides Nucleotides Nucleic Acids
33
291-295
2014
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2
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2
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6
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2
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-
694483
Joachims
Inhibition of deoxynucleoside ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
27
816-820
2008
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1
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1
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1
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674219
Sandrini
Nucleoside analogues are activ ...
Bacillus cereus
J. Antimicrob. Chemother.
60
510-520
2007
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1
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6
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1
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11
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1
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6
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3
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1
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1
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6
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1
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1
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6
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3
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675428
Welin
Structure-function analysis of ...
Mycoplasma mycoides
J. Mol. Biol.
366
1615-1623
2007
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1
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2
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2
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5
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1
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46
1
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1
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1
2
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2
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2
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1
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46
1
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-
-
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-
-
-
675444
Sandrini
Dictyostelium discoideum salva ...
Dictyostelium discoideum
J. Mol. Biol.
369
653-664
2007
-
-
1
1
-
-
-
5
-
-
1
1
-
7
-
-
-
-
-
-
-
-
10
1
-
-
-
6
-
-
-
1
-
-
-
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-
1
1
1
-
-
-
-
-
6
-
-
1
1
-
-
-
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-
-
-
-
10
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
690459
Sandrini
Deoxyribonucleoside kinases ac ...
Staphylococcus aureus
Antimicrob. Agents Chemother.
51
2726-2732
2007
-
-
1
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-
-
-
3
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1
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7
-
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3
1
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3
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1
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3
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1
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3
1
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3
-
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-
672159
Pol
Kinetic mechanism of deoxyaden ...
Mycoplasma mycoides
Biochemistry
45
513-522
2006
-
-
-
-
-
-
-
10
-
-
-
-
-
3
-
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6
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1
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1
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10
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6
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676722
Katahira
Profiles of purine biosynthesi ...
Solanum tuberosum
Planta
225
115-126
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
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-
1
1
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1
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1
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1
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1
1
-
1
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-
-
-
-
-
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-
-
-
-
-
-
-
-
660855
Krawiec
Inorganic tripolyphosphate (PP ...
Homo sapiens
Biochem. Biophys. Res. Commun.
301
192-197
2003
-
-
-
-
-
-
1
4
-
1
-
2
-
1
-
-
-
-
-
-
2
-
4
-
1
-
-
-
1
-
-
2
2
-
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-
-
2
-
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1
2
4
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1
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2
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2
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
662985
Krawiec
Ability of adenosine-2'(3')-de ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
22
1525-1529
2003
-
-
-
-
-
-
-
-
-
1
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2
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1
-
-
-
-
-
-
2
-
14
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7
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7
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1
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2
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2
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14
-
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-
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-
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-
-
640561
Andersen
Deoxynucleoside kinases encode ...
Bacillus subtilis
J. Biol. Chem.
276
5518-5524
2001
-
-
1
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1
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3
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1
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3
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1
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1
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1
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1
1
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1
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1
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3
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1
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1
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-
641988
Wang
Novel deoxynucleoside-phosphor ...
Acholeplasma laidlawii, Mycoplasma arginini, Mycoplasma mycoides, Mycoplasma pneumoniae
Mol. Microbiol.
42
1065-1073
2001
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-
1
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-
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7
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2
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12
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1
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10
1
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7
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1
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7
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2
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1
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10
1
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7
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-
641989
Park
-
Protein engineering of deoxynu ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Food Sci. Nutr.
6
79-81
2001
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3
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4
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3
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641984
Jenuth
Modeling of purine nucleoside ...
Mus musculus
Biochem. Cell Biol.
74
219-225
1996
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-
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1
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2
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1
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1
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1
1
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2
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641987
Szabados
Metabolism of adenosine and de ...
Homo sapiens
Int. J. Biochem. Cell Biol.
28
1405-1414
1996
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2
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1
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640565
Hong
Directed mutagenesis of deoxyg ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Biol. Chem.
270
6602-6606
1995
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4
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1
5
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6
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5
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640567
Ma
Cloning and expression of the ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Biol. Chem.
270
6595-6601
1995
1
-
1
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1
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2
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4
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1
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4
1
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1
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1
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1
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2
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1
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4
1
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640566
Ikeda
Heterodimeric deoxynucleoside ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
Biochemistry
33
5328-5334
1994
-
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-
1
4
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2
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2
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1
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2
2
1
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1
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4
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1
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1
2
2
1
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641937
Kierdaszuk
Selective inactivation of the ...
Homo sapiens
Biochemistry
29
4109-4114
1990
-
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-
1
1
1
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1
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1
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1
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2
3
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1
1
5
-
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1
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1
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1
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1
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1
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2
3
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1
1
5
-
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641980
Ikeda
Amino-terminal nucleotide-bind ...
Lactobacillus acidophilus
Biochemistry
27
8648-8652
1988
-
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3
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1
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1
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641089
Drabikowska
Purification and properties of ...
Rattus norvegicus
Z. Naturforsch. C
40
34-41
1985
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3
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2
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1
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2
-
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1
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-
-
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-
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-
-
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-
641099
Carson
Lymphospecific toxicity in ade ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
74
5677-5681
1977
-
-
-
-
-
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-
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1
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10
-
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10
-
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641950
Deibler
Deoxynucleoside kinases from L ...
Lactobacillus acidophilus
J. Biol. Chem.
252
8240-8244
1977
6
-
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2
1
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1
-
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1
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-
-
1
-
-
-
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2
-
1
-
-
-
-
-
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6
-
-
-
-
-
-
-
2
-
1
-
1
-
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-
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2
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1
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641977
Baxter
Purification and detection of ...
Bos taurus
Biochem. Soc. Trans.
5
979-981
1977
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1
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1
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641976
Streeter
The phosphorylation of ribavir ...
Rattus norvegicus
Biochemistry
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4543-4549
1974
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1
4
2
1
1
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4
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4
4
2
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1
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1
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1
1
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641981
Krygier
Mammalian deoxynucleoside kina ...
Bos taurus
J. Biol. Chem.
246
2745-2751
1971
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-
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1
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3
1
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1
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1
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1
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7
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1
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1
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1
1
7
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1
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1
1
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641974
Krygier
The regulatory properties of d ...
Bos taurus
Biochim. Biophys. Acta
161
578-580
1968
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1
11
1
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1
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1
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3
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1
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1
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11
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1
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1
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1
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