Protein Variants | Comment | Organism |
---|---|---|
A100V/R104M/D133A | 12fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
A100V/R104M/D133S | 6fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
A100V/R104M/D133T | ratio kcat/Km for deoxycytidine similar to wild-type | Homo sapiens |
D47E/R104Q/D133G/N163I/F242L | 1.5fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
additional information | R104 and D133 are key residues for substrate specificity | Homo sapiens |
R104M/D133A | 4fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
R104M/D133N | 3fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
R104M/D133S | 6fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
R104M/D133T | mutant with reversed substrate specificity, with elevated specific constant for thymidine phosphorylation and decreased activity for deeoxycytidine, deoxyadenosine, and deoxyguanosine | Homo sapiens |
R104Q/D133G | mutant is a generalist kinase with broader specificity and elevated turnover compared with wild-type | Homo sapiens |
R104Q/D133N | 1.5fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
deoxycytidine | wild-type, pH 7.5, 37°C | Homo sapiens | |
0.001 | - |
deoxycytidine | wild-type, pH 7.5, 30°C | Homo sapiens | |
0.00104 | - |
deoxycytidine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
0.00141 | - |
deoxycytidine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
0.00154 | - |
deoxycytidine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
0.002 | - |
deoxycytidine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
0.00388 | - |
thymidine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
0.00455 | - |
deoxycytidine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
0.00525 | - |
deoxycytidine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
0.00558 | - |
thymidine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
0.0058 | - |
thymidine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
0.0086 | - |
deoxycytidine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
0.0118 | - |
deoxycytidine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
0.0189 | - |
thymidine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
0.0209 | - |
deoxycytidine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
0.025 | - |
thymidine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
0.0256 | - |
thymidine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
0.0257 | - |
thymidine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
0.031 | - |
thymidine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
0.036 | - |
thymidine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
0.071 | - |
deoxycytidine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
0.074 | - |
thymidine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
0.079 | - |
2'-deoxyguanosine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
0.081 | - |
2'-deoxyadenosine | wild-type, pH 7.5, 37°C | Homo sapiens | |
0.081 | - |
2'-deoxyadenosine | wild-type, pH 7.5, 30°C | Homo sapiens | |
0.081 | - |
2'-deoxyguanosine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
0.091 | - |
2'-deoxyadenosine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
0.154 | - |
2'-deoxyguanosine | wild-type, pH 7.5, 37°C | Homo sapiens | |
0.155 | - |
2'-deoxyguanosine | wild-type, pH 7.5, 30°C | Homo sapiens | |
0.231 | - |
2'-deoxyadenosine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
0.24 | - |
2'-deoxyguanosine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
0.324 | - |
2'-deoxyguanosine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
0.398 | - |
2'-deoxyadenosine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
0.412 | - |
2'-deoxyadenosine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
0.452 | - |
2'-deoxyadenosine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
0.532 | - |
2'-deoxyadenosine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
0.598 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
0.691 | - |
2'-deoxyadenosine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
0.739 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
0.843 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
1.164 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
1.172 | - |
2'-deoxyguanosine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
1.203 | - |
2'-deoxyguanosine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
1.28 | - |
2'-deoxyguanosine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
1.364 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
1.437 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
3.485 | - |
thymidine | wild-type, pH 7.5, 37°C | Homo sapiens | |
3.485 | - |
thymidine | wild-type, pH 7.5, 30°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27707 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.5 | - |
substrate dideoxythymidine, wild-type, pH 7.5, 37°C | Homo sapiens |
26 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens |
28 | - |
substrate dideoxythymidine, mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens |
64 | - |
substrate dideoxythymidine, mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens |
88 | - |
substrate 2'-deoxy-3'-thiacytidine, wild-type, pH 7.5, 37°C | Homo sapiens |
108 | - |
substrate dideoxythymidine, mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens |
114 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens |
135 | - |
substrate dideoxythymidine, mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens |
149 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens |
186 | - |
substrate dideoxythymidine, mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens |
206 | - |
substrate dideoxythymidine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C | Homo sapiens |
270 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens |
545 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens |
599 | - |
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens |
815 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C | Homo sapiens |
984 | - |
substrate 1-beta-D-arabinosylcytosine, wild-type, pH 7.5, 37°C | Homo sapiens |
1387 | - |
substrate 1-beta-D-arabinosylcytosine, mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens |
1648 | - |
substrate gemcitabine, wild-type, pH 7.5, 37°C | Homo sapiens |
2262 | - |
substrate gemcitabine, mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens |
3177 | - |
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens |
3308 | - |
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens |
3339 | - |
substrate 1-beta-D-arabinosylcytosine, mutant R104M/D133SA, pH 7.5, 37°C | Homo sapiens |
4267 | - |
substrate gemcitabine, mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens |
4400 | - |
substrate 1-beta-D-arabinosylcytosine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C | Homo sapiens |
4530 | - |
substrate gemcitabine, mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens |
5624 | - |
substrate gemcitabine, mutant R104M/D133SA, pH 7.5, 37°C | Homo sapiens |
6009 | - |
substrate gemcitabine, mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens |
6460 | - |
substrate gemcitabine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-beta-D-arabinosylcytosine | - |
Homo sapiens | ADP + 1-beta-D-arabinosylcytosine 5'-phosphate | - |
? | |
ATP + 2'-deoxy-3'-thiacytidine | - |
Homo sapiens | ADP + 2'-deoxy-3'-thiacytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyadenosine | - |
Homo sapiens | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | - |
Homo sapiens | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + dideoxythymidine | - |
Homo sapiens | ADP + dideoxythymidine 5'-phosphate | - |
? | |
ATP + gemcitabine | - |
Homo sapiens | ADP + gemcitabine 5'-phosphate | - |
? | |
ATP + thymidine | - |
Homo sapiens | ADP + TMP | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
deoxycytidine | wild-type, pH 7.5, 37°C | Homo sapiens | |
0.064 | - |
deoxycytidine | wild-type, pH 7.5, 30°C | Homo sapiens | |
0.21 | - |
deoxycytidine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
0.31 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
0.37 | - |
deoxycytidine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
0.38 | - |
thymidine | wild-type, pH 7.5, 37°C | Homo sapiens | |
0.38 | - |
thymidine | wild-type, pH 7.5, 30°C | Homo sapiens | |
0.58 | - |
deoxycytidine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
0.68 | - |
thymidine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
1.01 | - |
thymidine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
1.08 | - |
2'-deoxyadenosine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
1.18 | - |
2'-deoxyguanosine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
1.2 | - |
deoxycytidine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
1.23 | - |
2'-deoxyguanosine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
1.3 | - |
deoxycytidine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
1.49 | - |
thymidine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
1.5 | - |
2'-deoxyguanosine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
1.51 | - |
thymidine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
1.57 | - |
thymidine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
1.7 | - |
deoxycytidine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
1.72 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
1.75 | - |
2'-deoxyadenosine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
1.88 | - |
thymidine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
1.91 | - |
thymidine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
1.92 | - |
2'-deoxyguanosine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
2 | - |
2'-deoxyadenosine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
2.1 | - |
deoxycytidine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.23 | - |
deoxycytidine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
2.27 | - |
thymidine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
2.29 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.33 | - |
thymidine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.5 | - |
2'-deoxyguanosine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.67 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.71 | - |
2'-deoxyadenosine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.86 | - |
deoxycytidine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
3.1 | - |
2'-deoxyadenosine | wild-type, pH 7.5, 37°C | Homo sapiens | |
3.1 | - |
2'-deoxyadenosine | wild-type, pH 7.5, 30°C | Homo sapiens | |
3.26 | - |
2'-deoxyguanosine | wild-type, pH 7.5, 37°C | Homo sapiens | |
3.26 | - |
2'-deoxyguanosine | wild-type, pH 7.5, 30°C | Homo sapiens | |
3.29 | - |
thymidine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
3.37 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
3.4 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
3.47 | - |
2'-deoxyadenosine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
3.54 | - |
deoxycytidine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
3.59 | - |
2'-deoxyguanosine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
4.14 | - |
2'-deoxyadenosine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
4.38 | - |
2'-deoxyguanosine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
4.85 | - |
2'-deoxyadenosine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens |