Activating Compound | Comment | Organism | Structure |
---|---|---|---|
RAB1 | activates PI4KIIIalpha and stimulates the production of phosphatidylinositol 4-phosphate at the Golgi | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
recombinant expression and co-localization of fluorescent-labeled GBF1 with the fluorescent-labeled PI4KIIIalpha in COS-7 cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | depletion of isozymes PI4KIIIalpha and PI4KIIIbeta using siRNA, depletion of PI4KIIIalpha prevents the recruitment of GBF1 to Golgi membranes,phenotypes, overview | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
LY294002 | - |
Saccharomyces cerevisiae | |
PAO | inhibitor of type III PI4Ks, with greater specificity for PI4KIIIalpha than for PI4KIIIbeta | Saccharomyces cerevisiae | |
Wortmannin | inhibitor of type III PI4Ks | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | PI4KIIIalpha | Saccharomyces cerevisiae | 5783 | - |
Golgi apparatus | PI4KIIIalpha | Saccharomyces cerevisiae | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | Saccharomyces cerevisiae | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | - |
Saccharomyces cerevisiae | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol 4-kinase | - |
Saccharomyces cerevisiae |
PI4KIIIalpha | - |
Saccharomyces cerevisiae |
type III PI4K | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | Inhibitors of phosphatidylinositol 4-phosphate synthesis or depletion of PI4KIIIalpha, a phosphatidylinositol 4-kinase localized to the endoplasmic reticulum and Golgi, prevents the recruitment of GBF1 to Golgi membranes | Saccharomyces cerevisiae |
physiological function | phosphatidylinositol 4-phosphate, synthesized by phosphatidylinositol 4-kinase isozyme PI4KIIIalpha, is required for recruitment of the guanine nucleotide exchange factor GBF1 to Golgi membranes, where it is bound and activates Arf1. Activated Arf1 is required for formation of trafficking vesicles in the Golgi apparatus, overview. Rab1 contributes to the specificity and timing of GBF1 recruitment by activating PI4KIIIalpha | Saccharomyces cerevisiae |