Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | optimal concentration is 0.1% | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
isoform PI4K92 is expressed as His6 tagged protein in Sf9 cells reaching a level of approximately 5% of cellular proteins | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | - |
Homo sapiens | |
Wortmannin | IC50: about 300 nM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
ATP | 37°C | Homo sapiens | |
1 | - |
phosphatidylinositol | 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UBF8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphoprotein, additionally phosphate is incorporated by incubation with ATP/Mg or ATP/Mn. Phosphorylation sites are mapped by MALDI-MS and LC-MS/MS at the following positions: S258, T263, S266, S277, S294, T423, S496, T504 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
type III kinase, isoform PI4K92 is expressed as His6 tagged protein in Sf9 cells | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.581 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.365 | - |
adenosine | 37°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0003 | - |
IC50: about 300 nM | Homo sapiens | Wortmannin |