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Literature summary for 2.7.1.63 extracted from
- Coevolution of both Thermostability and activity of polyphosphate glucokinase from Thermobifida fusca YX (2018), Appl. Environ. Microbiol., 84, e01224-18 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | great potentials of directed evolution in obtaining high-performance BioBricks suitable for the in vitro synthetic biology platform | Thermobifida fusca |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Thermobifida fusca |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A231T | 2.8fold increase in t1/2 at 55°C | Thermobifida fusca |
| F30L/R34P/T72A/V88M/A231T | 7200fold increase in t1/2 at 55°C | Thermobifida fusca |
| F30L/T72A/A231T | 185fold increase in t1/2 at 55°C | Thermobifida fusca |
| F30LT72A/V88M/A231T | 1570fold increase in t1/2 at 55°C | Thermobifida fusca |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (phosphate)n + D-glucose | Thermobifida fusca | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida fusca | Q47NX5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermobifida fusca |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 285.3 | - |
pH 7.5, 50°C | Thermobifida fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (phosphate)n + D-glucose | - |
Thermobifida fusca | (phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPGK | - |
Thermobifida fusca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
wild-type enzyme and mutant enzyme A231T | Thermobifida fusca |
| 55 | - |
mutant enzyme F30L/T72A/A231T | Thermobifida fusca |
| 60 | - |
mutant enzyme F30L/T72A/V88M/A231T | Thermobifida fusca |
| 65 | - |
mutant enzyme F30L/R34P/T72A/V88M/A231T | Thermobifida fusca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
t1/2: 1.4 min (wild-type enzyme), t1/2: 3.2 min (mutant enzyme A231T), t1/2: 240 min (mutant enzyme F30L/T72A/A231T), t1/2: 2200 min (mutant enzyme F30L/T72A/V88M/A231T), t1/2: 10080 min (mutant enzyme F30L/R34P/T72A/V88M/A231T) | Thermobifida fusca |
| 55.9 | - |
Tm-value, wild-type enzyme | Thermobifida fusca |
| 59.1 | - |
Tm-value, mutant enzyme A231T | Thermobifida fusca |
| 65.2 | - |
Tm-value, mutant enzyme F30L/T72A/A231T | Thermobifida fusca |
| 70.6 | - |
Tm-value, mutant enzyme F30L/T72A/V88M/A231T | Thermobifida fusca |
| 75.7 | - |
Tm-value, mutant enzyme F30L/R34P/T72A/V88M/A231T | Thermobifida fusca |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | coevolution of both the thermostability and catalytic activity of the enzyme by directed evolution | Thermobifida fusca |
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