Cloned (Comment) | Organism |
---|---|
gene kdgK as part of the ED gene cluster, expresssion in Escherichia coli | Saccharolobus solfataricus |
gene kdgK as part of the ED gene cluster, expresssion in Escherichia coli as soluble proein at low rate | Thermoproteus tenax |
Protein Variants | Comment | Organism |
---|---|---|
additional information | reconstruction of the Entner-Doudoroff pathway with recombinantly produced enzymes of Thermoproteus tenax and Solfolobus solfataricus, overview | Saccharolobus solfataricus |
additional information | reconstruction of the Entner-Doudoroff pathway with recombinantly produced enzymes of Thermoproteus tenax and Solfolobus solfataricus, overview | Thermoproteus tenax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.178 | - |
2-dehydro-3-deoxy-D-gluconate | pH 7.0, 70°C, recombinant enzyme | Thermoproteus tenax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely dependent on | Saccharolobus solfataricus | |
Mg2+ | absolutely dependent on | Thermoproteus tenax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, recombinant enzyme, SDS-PAGE, x * 33308, sequence calculation | Thermoproteus tenax |
33308 | - |
x * 32000, recombinant enzyme, SDS-PAGE, x * 33308, sequence calculation | Thermoproteus tenax |
Organism | UniProt | Comment | Textmining |
---|
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli, by heat precipitation and gel filtration | Thermoproteus tenax |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the archaeon grows optimally at 80-85°C and pH 2-4 | Saccharolobus solfataricus | - |
additional information | the archaeon grows optimally at about 90°C and pH 5.0 | Thermoproteus tenax | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharolobus solfataricus |
additional information | - |
- |
Thermoproteus tenax |
0.104 | - |
recombinant cell extract after heat precipitation step | Saccharolobus solfataricus |
41.3 | - |
purified recombinant enzyme | Thermoproteus tenax |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, recombinant enzyme, SDS-PAGE, x * 33308, sequence calculation | Thermoproteus tenax |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the archaeon grows optimally at 80-85°C and pH 2-4 | Saccharolobus solfataricus |
additional information | - |
the archaeon grows optimally at about 90°C and pH 5.0 | Thermoproteus tenax |
60 | - |
assay at | Saccharolobus solfataricus |
70 | - |
assay at | Thermoproteus tenax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the archaeon grows optimally at 80-85°C and pH 2-4 | Saccharolobus solfataricus |
additional information | - |
the archaeon grows optimally at about 90°C and pH 5.0 | Thermoproteus tenax |
7 | - |
assay at | Saccharolobus solfataricus |
7 | - |
assay at | Thermoproteus tenax |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | absolutely dependent on | Saccharolobus solfataricus | |
ATP | absolutely dependent on | Thermoproteus tenax |